中高温大曲中高产蛋白酶菌株的选育  

The Breeding of High Protease-producing Strain Isolated from Songhe Daqu

作　　者：李学思 侯小歌[2] 李绍亮 闫培训 张杰 胡炳义[2] 

机构地区：[1]河南省宋河酒业股份有限公司,河南鹿邑477265 [2]周口师范学院生命科学与农学学院,河南周口466000

出　　处：《酿酒》2017年第2期47-51,共5页Liquor Making

基　　金：河南省教育厅科学技术研究重点项目(编号14A180026);周口师范学院科研成果孵化专项基金资助项目(项目编号2011-zknufh-01)

摘　　要：从宋河大曲中共分离得到39株产蛋白酶芽孢菌株,采用透明圈法和摇瓶发酵法筛选出1株高产蛋白酶菌株MSPN-11,酶活达114.8U/m L。以该菌株为出发菌种,采用紫外诱变的育种方法,以致死率和蛋白酶活力为指标选育高产蛋白酶菌株。研究表明:紫外照射时间160s、照射距离50cm^55cm的剂量处理,菌株致死率达80%以上,且90%诱变菌株的R/r值在3.0以上,得到1株高产蛋白酶菌株MSPR 8,酶活提高了26.38%。39 protease-producing bacillus isolated from Songhe Daqu were obtained. The strain MSPN-11 which demonstrated high protease productivity （114.8 U/mL） was screened by observing transparent rings and measuring the protease activity of shake flask fermentation. The high protease-producing strain would be breeded using ultraviolet mutagenesis with protease activity and lethality rate of colonies as measuring indexes.The results showed that： lethality rate of colonies reached above 80% when UV irradiation time and distance was chose 160 s and 50 cm-55 cm, respectively. 90% of the mutant strain R/r values were above 3.0. Strain MSPR8 which showed higher protease activity increasing 26.38% was obtained by screening strains breeded using ultraviolet mutagenesis.

关 键 词：大曲 蛋白酶芽孢菌株 紫外诱变 选育 

分 类 号：TS262.3[轻工技术与工程—发酵工程] TS261.15[轻工技术与工程—食品科学与工程]