小粒咖啡褐斑病病原菌鉴定及田间抗病性研究  

Identification of Pathogen Causing Coffee Tree Brown Leaf Spot and Disease Resistance of Coffea arabica Varieties in Field

作　　者：龙亚芹[1] 段春芳[2] 刘杰[1] 彭海明 申科[1] 王雪松[1] 石华忠 罗梓文[1] 陈雷[1] 李荣福[4] 

机构地区：[1]云南省农业科学院茶叶研究所,云南勐海666201 [2]云南省农业科学院热带亚热带经济作物研究所,云南保山678000 [3]云南爱伲农牧(集团)有限公司,云南普洱665000 [4]云南省农业科学院农业经济与信息研究所,云南昆明650205

出　　处：《西南林业大学学报（自然科学）》2017年第2期152-157,共6页Journal of Southwest Forestry University：Natural Sciences

基　　金：云南省技术创新人才培养对象项目(052)资助;国际科技合作与交流专项(2014DFA32230)项目资助

摘　　要：对咖啡褐斑病病原菌进行分离培养和致病性测定,并结合形态学和分子鉴定,确定其病原菌,同时开展咖啡褐斑病田间发生情况和品种抗病性调查研究。结果表明:从不同地点采集的小粒咖啡病叶上分离出3个菌株,其形态特征相同。选取从云南(CCYN05)和海南(CCHN05)样品上纯化到的2个菌株,对其rDNA-ITS克隆测序分析,2个菌株为同一致病菌。结合形态学特征,将该病原菌鉴定为咖啡生尾孢。调查了5个小粒咖啡品种对褐斑病的田间抗性,没有发现免疫品种和抗病品种,5个品种抗病性由强至弱依次为卡杜埃瑞吉纳、卡杜拉、卡杜埃44、卡蒂膜、维拉萨奇。Pathogen of coffee tree brown leaf spot were identified by means of isolation culture, pathogenicity test, and the combination of morphology and molecular biology identification. At the same time, the investigation of occurrence and varieties resistance on coffee brown spot disease were carried out. The results showed that morphological characteristics of 3 pathogenic strains from coffee leaf samples in different places were the same. CCYN05 and CCHN05 were purified, and 2 strains were the same pathogen by rDNA-ITS sequence alignment and analysis. And combined with morphology characteristics, the results revealed that this disease was caused by Cercospora coffeicola. The brown spot disease resistances of 5 Arabica coffee tree varieties were investigated in the field, there were no immune varieties and diseaseresistant varieties. The disease resistances sequences were as following： Catuaí Regional, Caturra, Catuaí44, Catimor, Villa Sarchí.

关 键 词：小粒咖啡 褐斑病 病原菌 抗病性 品种 

分 类 号：S763.15[农业科学—森林保护学]