内生真菌MG-9的主物质麦角甾醇的分离鉴定及抗氧化活性分析  

Isolation and Identification of Main Substance Ergosterol from the Endophytic Fungus MG-9 and Its Antioxidant Activity Analysis

作　　者：秦王阁阁 孔玉珊 蒋维[1] 李辉[1] 薛艳红[1] 刘呈雄[1] 刘士平[1] 

机构地区：[1]三峡大学生物与制药学院,湖北宜昌443002

出　　处：《三峡大学学报（自然科学版）》2017年第2期108-112,共5页Journal of China Three Gorges University：Natural Sciences

基　　金：国家自然科学基金项目"三峡河岸带植物疏花水柏枝内生菌介导的酚酸类物质变化及抗涝害胁迫机理研究"(31270389);国家自然科学基金应急管理项目"疏花水柏枝内生真菌RX01调控细胞热激蛋白介导的抗氧化胁迫能力研究"(31540065)

摘　　要：从疏花水柏枝中的一株内生真菌MG-9的粗提物中重结晶出一种主物质,采用NMR、MS等现代波谱学技术鉴定这种主物质为麦角甾醇.以LDH(乳酸脱氢酶)、SOD(超氧化物歧化酶)、caspase 3、caspase 9的酶活表达情况为指标,研究了MG-9主物质对神经细胞SH-SY5Y的毒害作用及抗氧化保护作用机理.结果表明:麦角甾醇在6.25-25μg/mL时对神经细胞SH-SY5Y毒性的量效关系不明显,而在6.25μg/mL时对神经细胞SH-SY5Y的氧化损伤保护具有更好的效果,将神经细胞SH-SY5Y在H_2O_2(800μmol/L)胁迫下的相对存活率提高了74.5%.并且麦角甾醇可通过抑制凋亡蛋白caspase 3的活性来达到对神经细胞SH-SY5Y的抗氧化保护作用.A main substance was recrystallized from the crude extract of an endophytic fungus MG-9 in Myricaria laxiflora, and the main substance was identified as ergosterol by modern spectroscopy techniques such as NMR and MS. The toxic effects and antioxidant protection mechanism of MG-9 main substance on the neu-rotoxicity of nerve cell SH-SY5Y were studied by using LDH （Lactic dehydrogenase）, SOD （Superoxide dismutase）, caspase 3 and caspase 9 enzyme activity expression as indicators. The results showed that the doseeffect relationship of ergosterol to nerve cell SH-SY5Y neurotoxicity was not significant at 6.25-25 μg/mL, but it had better effect on oxidative damage protection of nerve cell SH-SY5Y at 6.25μg/mL. The relative survival rate of nerve cell SH-SY5Y was increased by 74.5 % under H202 （800μmol/L）. And ergosterol can inhibit the activity of apoptosis protein caspase 3 to achieve anti-oxidative protection effect of nerve cell SH-SY5Y.

关 键 词：内生真菌 麦角甾醇 神经细胞保护 抗氧化活性 

分 类 号：R284.1[医药卫生—中药学]