一株产海藻糖合酶假单胞菌菌株的分离及鉴定  

Screening and identification of trehalose synthase-producing Pseudomonas sp.

作　　者：王一雯[1,2] 权淑静 马焕 陈国参[1] 刘德海[1] WANG Yiwen QUAN Shujing MA Huan CHEN Guocan LIU Dehai(Institute of Biology Co., Ltd., Henan Academy of Science, Zhengzhou 450008, China Henan Engineering Research Center of Industrial Enzymes, Zhengzhou 450008, China)

机构地区：[1]河南省科学院生物研究所有限责任公司,河南郑州450008 [2]河南省工业酶工程技术研究中心,河南郑州450008

出　　处：《中国酿造》2017年第4期58-62,共5页China Brewing

基　　金：2015年河南省科技创新杰出人才项目(154200510025)

摘　　要：由薄层层析法检测试验发现,从昆明磷矿粉中分离出的一株真细菌,其粗酶液能将麦芽糖异构化为海藻糖。通过显微观察发现,该菌株为革兰氏阴性菌,无色、杆状、具有鞭毛;菌体直径大小0.6~0.8μm,长度约1.4~2.0μm;胞内没有聚-β-羟丁酸(PHB)包含体;最佳生长温度为30℃,最适生长pH为7.0。经16S rRNA序列比对、DNA(G+C)mol%和DNA-DNA杂交等分子生物学技术以及生理生化特性检测后,认定该菌株为假单胞菌属(Pseudomonas sp.)一种,将其命名为BIE-1。该菌株增加了假单胞菌属菌种多样性,为海藻糖生物合成真细菌菌株提供了新的选择。A eubacterium was isolated from ground phosphate rock in Kunming, the detection results of thin layer chromatography （TLC） showed that the strain crude enzyme liquid could iosmerize maltose heterogeneous into trehalose. Through microscopic observation, the strain was gram-negative bacterium, colourless, rod-shaped, with a flagellum, 0.6-0.8μm in diameter and about 1.4-2.0μm in length. No accumulation of poly-[3-hydroxybu- tyrate （PHB） granules as inclusion bodies was observed. The optimal growth temperature of the strain was 30℃ and the optimal pH was 7.0. The strain was identified as Pseudomonas sp. and named BIE-1 by biological techniques （including 16S rRNA sequence analysis, DNA （G＋C） mol%, DNA-DNA hybridization, etc.） and physiological-biochemical characteristic. It increased the species diversity of Pseudomonas sp., which provided new choice for the trehalose biosynthesis by eubacteria.

关 键 词：假单胞菌属 16S RRNA DNA-DNA杂交 薄层层析法 海藻糖 

分 类 号：Q785[生物学—分子生物学]