番茄红素基因工程菌多酶调控研究进展  

Research progress in multi-enzyme regulation of genetically engineered bacteria producing lycopene

作　　者：徐加莉 左思仪 谢承佳[2] 江凌[3] 李霜[1] 黄和[4] 徐娴[4] Jiali Xu Siyi Zuo Chengjia Xie Ling Jiang Shuang Li He Huang and Xian Xu(State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, Jiangsu, China College of Chemical Engineering, Yangzhou Polytechnic Institute, Yangzhou 225127, Jiangsu, China State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, Jiangsu, China College of Pharmacy, Nanjing Tech University, Nanjing 211816, Jiangsu, China)

机构地区：[1]南京工业大学生物与制药工程学院材料化学工程国家重点实验室,江苏南京211816 [2]扬州工业职业技术学院化学工程学院,江苏扬州225127 [3]南京工业大学食品与轻工学院材料化学工程国家重点实验室,江苏南京211816 [4]南京工业大学药学院,江苏南京211816

出　　处：《生物工程学报》2017年第4期552-564,共13页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(No.21406111);江苏省自然科学基金(No.BK20130917);大学生创新与实验开放基金(No.2016DC365)资助~~

摘　　要：作为类异戊二烯化合物中四萜的代表性产品番茄红素,在生物体中是典型的多酶参与催化的反应产物,在2-甲基-D-赤藓糖醇-4-磷酸盐(MEP)和甲羟戊酸(MVA)合成途径中起着至关重要的作用。从番茄红素在原核和真核中的多酶合成途径出发,针对番茄红素合成途径的各种优化策略,首先介绍了多酶合成中的常规调控方法,包括多基因共表达质粒构建、基因顺序调控、启动子与核糖体结合位点调控及基因敲除和替换等方法。之后介绍了一些新型的多酶调控方法,包括多片段组装技术、人工支架自组装方法等。最后重点介绍了这些多酶调控方法在番茄红素合成中的应用。这些多酶合成调控方法为构建高产番茄红素菌株提供了极大的启发和研究基础。Lycopene plays a crucial role in the biosynthesis pathway of 2-methyl-derythritol-4-phosphate （MEP） and mevalonic acid （MVA）. It is a representative product of isoprenoid family, and a typical product of multi-enzyme catalytic reaction in organism. In this paper, we first introduced the general regulation methods in multi-enzyme synthesis reaction, including the construction of multi gene co-expression plasmid, gene order regulation, promoter and ribosome binding site regulation, gene knockout and replacement, aiming at the optimization strategies of multi-enzyme catalytic reaction in lycopene synthesis pathway. Meanwhile, we introduced several new regulation methods in multi-enzyme reaction, including multi-fragment assembly technology, artificial scaffold self-assembly methods and so on. At last, we summarized the application of these multi-enzyme regulation methods in lycopene synthesis. These methods provide a great inspiration and research foundation for the construction of lycopene-producing strains with high yield.

关 键 词：番茄红素 多酶调控 支架 基因工程 多片段组装 

分 类 号：O629[理学—有机化学] Q78[理学—化学]