Akt和SRC在Exosomes促进同源肺癌细胞迁移中的作用  

Role of Akt and SRC Pathways in Exosome-mediated Migration of Homologous Lung Adenocarninoma Cells

作　　者：解世林 曲晶磊[2] 范一博[2] 车晓芳[2] 侯科佐[2] 曲秀娟[2] 刘云鹏[2] 王晓楠[1] 康健[3] 胡雪君[1] 

机构地区：[1]中国医科大学附属第一医院呼吸疾病研究所老年病呼吸感染科,沈阳110001 [2]中国医科大学附属第一医院肿瘤内科辽宁省抗肿瘤药物与生物治疗重点实验室,沈阳110001 [3]中国医科大学附属第一医院呼吸疾病研究所呼吸内科,沈阳110001

出　　处：《中国医科大学学报》2017年第4期294-297,共4页Journal of China Medical University

基　　金：国家自然科学基金(81372546;81472193);辽宁省科学技术计划(2014226033;2014225013;L2014296);辽宁省教育厅重点实验室基础研究(LZ2014037)

摘　　要：目的研究肺癌细胞来源的Exosomes对其分泌细胞及同源肿瘤细胞迁移的影响,初步探讨PI3K/Akt和SRC信号转导通路在此过程中的作用。方法采用密度梯度离心法,从肺癌细胞A549的上清液中分离出肺癌细胞来源的Exosomes,利用透射电子显微镜观察Exosomes的形态,用Western blotting实验观察蛋白的表达。Transwell实验检测细胞的迁移能力。结果透射电子显微镜下观察肺癌A549细胞来源的Exosomes,其具有特征性的盘状结构,由双层膜构成,直径30~100 nm。Western blotting结果显示Exosomes表面富含CD9分子。A549细胞来源的Exosomes以剂量依赖性的方式促进A549和其同源肿瘤细胞HCC827细胞的迁移,并伴随Akt和SRC的活化。结论肺癌细胞来源的Exosomes能促进其分泌细胞和同源肿瘤细胞的迁移,其机制可能与Akt和SRC的活化有关。Objective To investigate the effect of Exosomes derived from lung cancer cells on the migration of secretory cells and homologous tumor cells and to explore the role of PI3K/Akt and SRC signaling pathways in this process. Methods Exosomes were isolated from the supernatant post density gradient centrifugation of A549,lung cancer cells. Morphology of the Exosomes was studied using transmission electron microscopy.Protein expression was analyzed using Western blotting. Cell migration was analyzed by a transwell assay. Results The double-membrane-bound Exosomes appeared as discal-shaped structures,30-100 nm in diameter. Western blotting showed that CD9 was abundant in the Exosomes. The Exosomes promoted the migration of A549 cells and their homologous tumor cells,HCC827 in a dose-dependent manner,accompanied by the activation of Akt and SRC. Conclusion The Exosomes derived from A549（lung cancer）cells promote the migration of the secreting cells and the homologous tumor cells.The mechanism may be correlated with the activation of Akt and SRC.

关 键 词：EXOSOMES 迁移 肺癌 AKT SRC 

分 类 号：R734.2[医药卫生—肿瘤]