Notch1通路与胶质瘤细胞管道形成能力相关性研究  

The study on Notch1 pathway-related tubular formation of glioma cell lines

作　　者：张清平[1] 梅鑫[2] 柯超[2] 陈银生[2] 陈芙蓉[2] 陈建良[3] 陈忠平[2] 

机构地区：[1]深圳市南山区人民医院神经外科,深圳518033 [2]中山大学肿瘤防治中心神经外科 [3]中山大学附属第八医院深圳市福田区人民医院神经外科

出　　处：《中国神经精神疾病杂志》2017年第2期116-119,共4页Chinese Journal of Nervous and Mental Diseases

基　　金：国家自然科学基金(编号:81372685);国家重点基础研究发展计划(973计划)(编号:2015CB755505);广东省自然科学基金(编号:S2013040012894);广东省科技计划项目(编号:2013B021800067,2016A02013004);深圳市科技创新委员会项目(编号:JCYJ20140416094330210);广东省医学科研基金(编号:A2014247)

摘　　要：目的探索胶质瘤细胞来源的管道(glioma clls derived vessels,GCDV)的形成机制。方法将胶质瘤细胞株U87、U251、U373、SF295、T98G、SKMG-4和C6进行体外三维培养,观察其管道形成能力。Western blot检测各个胶质瘤细胞株Notch1、Dll4蛋白的表达情况。结果三维培养C6细胞单个视野下(100×)的平均管道数(25.2±5.0)个,U373为(36.4±3.20)个,U87为(19.0±2.2)个,T98G为(12.6±2.4)个,SF295为(4.0±2.)个,U251为(0.2±0.4)个,SKMG-4为0。Notch1在U87、U251、T98G、SF295、SKMG-4、C6、U373表达相关密度分别为0.34、0.21、0.79、0.04、0.28、1.75、1.19,与管道形成能力显著相关(r=0.778,P=0.019);Dll4表达相关密度与管道形成能力不相关(r=0.635,P=0.062)。结论 Notch1蛋白表达与细胞株管道形成能力密切相关,而Dll4蛋白的表达与细胞株管道形成能力有待进一步探索。Objective To explore the mechanism of the formation of glioma cells derived vessels （GCDV）. Methods The tubular formation assay was performed on 3D cell cultures of U87,U251, U373, SF295, T98G, SKMG-4 and C6 glioma cell lines. The expression of Notchl ,Dll4 were examined by western blot analysis. Result The mean number of vasculogenie channels of cell lines C6, U373, U87, T98G, SF295, U251 and SKMG-4 per area （100×） were 25.2±5.0, 36.4±3.2, 19.0±2.2, 12.6±2.4, 4±2.2, 0.2±0.4 and 0, respectively. The relative protein densities of Notchl in U87, U251, T98G, SF295, SKMG-4, C6 and U373 were 0.34, 0.21, 0.79,0.04, 0.28, 1.75 and 1.19, which were significantly related with the tubular formation ability （P〈0.05）. However, the expression of Dll4 was not associated with tubular formation ability （P〉0.05）. Conclusion Notchl may play a key role in tubular formation of glioma cells while the role of Dll4 in the process needs further study.

关 键 词：NOTCH1 胶质瘤 三维培养 管道形成 

分 类 号：R739.41[医药卫生—肿瘤]