HPLC测定菠萝汁中多菌灵、噻菌灵和甲基硫菌灵  

Determination of Residuals of Carbendazim,Thiabendazole and Thiophanate-methyl in Pineapple Juice by HPLC

作　　者：李涛[1] 李文辉 李俊毅 李宨妍 利光辉 庄濠宇 

机构地区：[1]揭阳职业技术学院,揭阳522051 [2]揭阳市质量计量监督检测所,揭阳522000 [3]揭阳出入境检验检疫局,揭阳522000

出　　处：《食品工业》2017年第4期289-291,共3页The Food Industry

基　　金：揭阳市科技计划项目(201407);揭阳职业技术学院2014学年度科研项目(JYCZZCX031513)

摘　　要：建立了结合固相萃取,利用高效液相色谱同时检测菠萝汁中多菌灵、噻菌灵和甲基硫菌灵残留量的分析方法。结果表明,以甲醇-水作为流动相,经梯度洗脱后,3种农药可以较好地分离。3种农药的质量浓度在0.10~2.00μg/m L范围内具有良好的线性关系,该方法的定量限分别为0.164,0.155和0.165 mg/L,加标回收率为79.1%~108.2%,相对标准偏差为1.6%~10.8%,方法灵敏度、精密度较高,适合菠萝及菠萝汁中多菌灵、噻菌灵和甲基硫菌灵农药残留的检测。HPLC combined solid phase extraction was applied to the determination of residuals of carbendazim, thiabendazole and thiophanate-methyl in pineapple juice. The results showed that three pesticides were separated by gradient elution with methanol-water as the mobile phase. There were good linear relationships between values of peak areas and concentrations of the three pesticides in the range of 0.10-2.00μg/mL. Their limits of quantification were 0.164, 0.155 and 0.165 mg/L and the average spike recoveries of three pesticides were 79.1%-108.2%, with RSDs in the range of 1.6% to 10.8%. The method could be used for the detection of carbendazim, thiabendazole and thiophanate-methyl in pineapple and pineapple juice with high sensitivity and precision.

关 键 词：高效液相色谱 菠萝汁 多菌灵 噻菌灵 甲基硫菌灵 

分 类 号：O657.72[理学—分析化学]