Streptomyces sp.Tü 4128中新型抗生素bagremycins抗性基因bagJ的研究  

Identification of bagJ as A Resistant Gene for Novel Antibiotic Bagremycins in Streptomyces sp.Tü 4128

作　　者：张玉琛[1] 吴海珍[1] 鞠诚 祁双双 叶江[1] 张惠展[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《华东理工大学学报（自然科学版）》2017年第2期184-192,共9页Journal of East China University of Science and Technology

基　　金：国家自然科学基金(31200026)

摘　　要：通过框内敲除鉴定了链霉菌Streptomyces sp.Tü4128中基因bagJ的功能。HPLC结果表明,敲除基因bagJ导致抗生素生产水平大幅降低;回补菌株恢复了抗生素的生产;过表达菌株大幅提高了抗生素的产量,证明基因bagJ在新型抗生素bagremycins的生物合成中必不可少。抑菌圈实验表明基因bagJ敲除菌株较野生菌株对bagremycins更加敏感;BagJ在Streptomyces lividans TK64中异源表达后使该菌株对bagremycins的耐受性增强,证明bagJ是新型抗生素bagremycins的抗性基因。扫描电镜的结果证明了BagJ异源表达后导致Streptomyces TK64的菌丝形态发生了改变。其他抗生素的敏感性实验表明,BagJ可能是一个逆向转运蛋白。T h e fu n c t io n of gene bagj via in- fram e d elet io n in Streptomyces sp. T i i 4128 was identified. The resul t of HPLC analysis showed that the production of bagremycins was s ignif icantly decreased in the single knock-out strain of bagj. T h e p ro d u c tio n of b ag rem y c in s was reco v ered in th e com p lem en tat io n s train. The overexpres s ion of gene bagj re su l te d in p ro d uc in g fa r m o re b a g rem y c in s , w h ich ind icated th a t bagj was in d isp en sab le in the b iosynthe s is of bag remyc in s . The te s t of in h ib i t io n zones sh owed that th e bagj muta nt was mo re s e nt i t ive to b ag remy c in s tha n the wi ld type. He te roge nous ex p re s s io n of Bagj in Streptomyces lividans TK6 4 en hanced i ts en d u ran ce to b ag rem y c in s which su g g e s ted th a t bagj m ig h t be a resistant gene of the novel ant ibiot ics bagremycins . SEM showed that the morphology of the mycelia was changed in the heterogenous expres s ion strain of Bagj in S. l ividams TK64. The sensi t ivi ty as says of other ant ibiot ics showed that Bagj was probably an ant ipor ter .

关 键 词：bagremycins STREPTOMYCES sp.Tü 4128 bagJ MFS家族 抗性基因 

分 类 号：Q71[生物学—分子生物学]