水解珍珠体外抗氧化活性的研究  

Study on antioxidant activities of pearl hydrolyzate in vitro

作　　者：蒲月华[1] 邓旗[1] 何锦锋 童银洪[1] 

机构地区：[1]广东海洋大学珍珠研究所,广东湛江524025 [2]北海市产品质量检验所,广西北海536000

出　　处：《化学研究与应用》2017年第4期555-559,共5页Chemical Research and Application

基　　金：广东省海洋渔业科技推广专项(A201208A05)资助;国家质检总局科技计划项目(2009QK386;2011QK337)资助

摘　　要：本文以L-抗坏血酸(VC)为对照组,测定水解珍珠对DPPH自由基、羟基自由基、超氧阴离子自由基和ABTS自由基的清除能力,研究水解珍珠的体外抗氧化活性。结果表明:水解珍珠对DPPH和ABTS自由基具有较强的清除能力,IC_(50)分别为0.07%和0.14%;而对羟基自由基和超氧阴离子自由基的清除能力相对较弱,IC_(50)分别为12.60%和7.65%;其IC_(50)均低于VC,差异具有统计学意义(p<0.05)。另外,水解珍珠对DPPH自由基、羟基自由基、超氧阴离子自由基和ABTS自由基的清除率都随着体积分数的增大而显著增加(p<0.05)。水解珍珠具备较高的抗氧化能力,可为珍珠资源的开发利用提供理论依据。The scavenging capacities of pearl hydrolyzate on DPPH·,OH·,O_2^-·and ABTS·were assayed in contrast with L-Ascorbic Acid(VC) to investigate the antioxidant effects of pearl hydrolyzate in vitro. The experimental results indicated that pearl hydrolyzate had strong effect of scavenging on DPPH·and ABTS·,which the IC_(50) were 0. 07% and 0. 14%,respectively. And the scavenging ability of pearl hydrolysate on OH·and O_2^-·were relatively weaker,which the IC_(50) were 12. 60% and 7. 65%,respectively.The IC_(50) of pearl hydrolysate were lower than VC and the differences were significant(p<0. 05). Moreover,with the increase of the volume fraction of pearl hydrolyzate,the scavenging rate of DPPH·,OH·,O_2^-·and ABTS·increased significantly(p<0. 05). Therefore,pearl hydrolysate has strong antioxidant capacity,and it provides a theoretical foundation for the development and utilization of pearl resources.

关 键 词：水解珍珠 自由基 抗氧化活性 

分 类 号：O657.3[理学—分析化学]