Aurora家族基因在急性白血病细胞中的表达及其临床意义  

Expression of Aurora Family Genes in Acute Leukemia and Its Clinical Significance

作　　者：刘自磊[1] 邢莹[2] 李涛[3] 王冲[1] 刘素芳[2] 赵华延 张荣辉[1] 马亚静[4] 张鑫烨 田文亮[1] 刘柳[1] 孙慧[1] 孙玲[1] 

机构地区：[1]郑州大学第一附属医院血液科,河南郑州450052 [2]郑州大学医学科学院,河南郑州450052 [3]郑州大学第一附属医院血液研究室,河南郑州450052 [4]郑州大学第一附属医院郑州大学医学科学院,河南郑州450052

出　　处：《中国实验血液学杂志》2017年第2期334-339,共6页Journal of Experimental Hematology

基　　金：国家自然科学基金(813000418);河南省科技厅攻关项目(132102310078)

摘　　要：目的:研究Aurora-A、B、C(AUR-A、B、C)基因mRNA在急性白血病中的表达情况及其与临床指标的相关性。方法:采用荧光定量PCR检测73例初诊急性白血病(AL)患者(初诊组)、20例化疗完全缓解急性白血病患者(缓解组)和骨髓单个核细胞中AUR-A、B、C mRNA表达水平,14例健康志愿者作为对照(健康组),并分析它们分别在不同分组中的表达性差异,与临床指标的相关性及AUR-A、B、C mRNA表达水平之间的相关性。结果:AURA、B、C mRNA表达在初诊组均高于健康组和缓解组(P<0.01),健康组和缓解组3种基因mRNA表达水平差异均无统计学意义(P>0.05),AUR-A、B、C 3种基因mRNA表达水平在急性淋巴细胞白血病(ALL)组均高于急性髓系白血病(AML)组(P<0.01);AUR-A、B、C mRNA表达在高危组均高于低危组(P<0.05),在高危组与中危组及中危组与低危组之间差异无统计学意义(P>0.05);AUR-A、B、C表达在CD34、CD71、CD56阴性组和阳性组差异无统计学意义(P>0.05),在不同性别、染色体、1疗程化疗是否缓解分组中差异无统计学意义(P>0.05)。73例初诊AL患者中,AUR-A mRNA表达水平与初诊乳酸脱氢酶(LDH)水平和危险分层呈显著正相关(r=0.279,P=0.017;r=0.314,P=0.007);AUR-B与初诊LDH水平和危险分层呈显著正相关(r=0.277,P=0.018;r=0.349,P=0.002),AUR-A、B均与年龄、初诊白细胞(WBC)水平、初诊骨髓幼稚细胞比例和1疗程是否缓解无显著相关性;AUR-C表达水平与初诊白细胞水平、初诊LDH水平和危险分层显著正相关(r=0.263,P=0.025;r=0.348,P=0.003;r=0.376,P=0.001),与年龄显著负相关(r=-0.241,P=0.040),而与初诊骨髓中幼稚细胞比例和1疗程是否缓解无显著相关性;AUR-A和B(r=0.444,P=0.000)、AUR-B和C(r=0.763,P=0.000)、AUR-A和C(r=0.616,P=0.000)mRNA表达水平均呈显著正相关。结论:AUR-A、B、C mRNA在初诊急性白血病患者中高表达并且三者间表达水平显著正相关,其高表达与白血病类型、危险分层、初�Objective： To explore the mRNA expression of Aurora-A,B, C（AUR-A,B, C） in acute leukemia（AL） and their correlations with the clinical indications. Methods： The mRNA expression levels of A UR-A, B, C in 73 cases of newly diagnosed AL （untreated group）, 20 cases of AL with remission （remission group） and 14 healthy volunteers as control （healthy group） were detected by QRT-PCR, and the difference of expression levels in difference groups, their correlations with clinical indicators and the correlation between the AUR-A, B, C mRNA expression levels themselves were analyzed. Results： The mRNA expression levels of AUR-A,B, C in untreated group were all higher than those in healthy group and remission group （ P 〈 0.01 ）, but there was not significant difference between healthy group and remission group（ P 〉 0.05 ） ; the mRNA expressions of AUR-A ,B, C in acute lymphoblastic leukemia（ALL） group were all significantly higher than that in AML group（ P 〈 0.01 ）. The mRNA expression of AUR-A, B, C in high risk group was higher than that in low risk group（P 〈0.05）, but there was no difference in mRNA expression of AUR-A,B,C between high risk group and middle risk group as well as between middle risk group and low risk group （ P 〉 0.05 ）. The mRNA expression of AUR-A, B, C in CD34, CD71 and CD56 negative group was not statistically different from that in CD34, CD71 and CD56 positive group（P 〉 0.05 ）. In 73 cases of newly diagnosed AL, the mRNA expression levels of AUR-A, B significantly were positively correlated with lactate dehydrogenase（LDH） level and risk stratification （r = 0.279, P=0.017; r=0.314, P =0.007 and r =0.277, P=0.018; r =0.349, P=0.002）, while the mRNA expression levels of AUR-A, B were not significantly correlated with age, WBC count, blast ratio in bone marrow at initial diagnosis and remission or no-remission after 1 cours of chemotherapy; the mRNA expression level of AUR-C was significantly positively correlated with WBC count （ r

关 键 词：急性白血病 Aurora家族基因 AURORA-A AURORA-B Aurora-C 

分 类 号：R733.71[医药卫生—肿瘤]