QuEChERS/HPLC/DAD法同时检测果蔬中多种植物激素残留  

Simultaneous Determination of Different Phytohormones Residues in Fruits and Vegetables by QuEChERS/DAD/HPLC

作　　者：朱松[1] 袁晓晴[2] 戴军[1] 陈尚卫[1] 

机构地区：[1]江南大学食品科学与技术国家重点实验室,江南大学分析测试中心,江苏无锡214122 [2]河南牧业经济学院,河南郑州450000

出　　处：《分析测试学报》2017年第4期513-518,共6页Journal of Instrumental Analysis

基　　金：食品科学与技术国家重点实验室自主科研课题;高等学校重点科研项目(15A550015);国家自然科学基金(31401532)

摘　　要：采用高效液相色谱法,建立了同时分析玉米素(Z)、赤霉酸(GA)、多效唑(PBZ)、4-氟苯氧乙酸(4-FPA)、4-氯苯氧乙酸(4-CPA)、吲哚-3-乙酸(IAA)、吲哚-3-丁酸(IBA)、6-苄氨基嘌呤(6-BA)、脱落酸(ABA)、萘乙酸(NAA)、氯吡脲(CPPU)、2,4-二氯苯氧乙酸(2,4-D)及2,4,5-三氯苯氧乙酸(2,4,5-T)13种植物激素含量的方法。采用含0.5%甲酸的80%乙腈进行提取,分散固相萃取吸附剂(C18和硅藻土)进行净化,选取Waters XBridge C_(18)色谱柱,以乙腈-水为流动相进行梯度洗脱,二极管阵列检测器200~400nm检测,外标法定量。结果表明,13种植物激素在50 min内可实现基线分离,在线性范围内的相关系数(r)为0.992 1~0.999 3;加标回收率为68.4%~95.1%;相对标准偏差(RSD)均小于5%;方法的检出限为0.005~0.020 mg/kg;定量下限为0.01~0.09 mg/kg。该方法前处理操作快速、简便,具有良好的灵敏度、精密度和回收率,适用于果蔬的质量监控。This work describes the development of a sensitive and reliable method using QuEChERS sample preparation and high performance liquid chromatography-diode array detector（HPLC-DAD） for simultaneous determination of phytohormones, zeatin（Z）, gibberellic acid（GA）, paclobutrazol（PBZ）, 4-fluorophenoxyacetic acid（4-FPA）, 4-chlorophenoxyacetic acid（4-CPA）, 3-indolyl-acetic acid（IAA）, 3-indolyl-butyric acid（IBA）, 6-benzylaminopurine（6-BA）, abscisic acid（ABA）, 1-naphthyl acetic acid（NAA）, forchlorfenuron（CPPU）, 2,4-dichlorophenoxyaceticacid（2,4-D）, 2,4,5-trichlorophenoxyacetic acid（2,4,5-T）, in fruits and vegetables.The samples were extracted with 80% acetonitrile containing 0.5% acetic acid,and the extract was cleaned up with dispersive solid phase extraction adsorbent（powder of C18 and diatomaceous earth）.The phytohormones were separated on a Waters XBridge C18,and eluated utilizing a gradient elution program of acetonitrile and water containing 0.05% acetic acid（by volume） as mobile phase.The correlation coefficients of calibration curves were over 0.99 in the corresponding concentration ranges.The average recoveries of the 13 analytes at three spiked concentration levels varied from 68.4% to 95.1% with relative standard deviations（RSD） less than 5%.The limits of detection（LOD） and quantitation（LOQ） were in the ranges of 0.005-0.020 mg/kg and 0.01-0.09 mg/kg,respectively.The real sample tests showed that the proposed method was simple,rapid,reliable and cost-effective,and was suitable for the simultaneous determination of phytohormones in fruits and vegetables.

关 键 词：QUECHERS 高效液相色谱(HPLC) 植物激素 果蔬 

分 类 号：O657.72[理学—分析化学] O629.8[理学—化学]