孕期高果糖摄入对胚胎发育和胎盘血管因子的研究  

Effects of High Fructose Consumption During Pregnancy on Embryonic Development and Placental Vascular Factors

作　　者：赵迪迪 韩磊[2] 贺娟[2] 梁惠[1] 马爱国[1] 肖子义 

机构地区：[1]青岛大学公共卫生学院,山东青岛266021 [2]青岛大学附属医院,山东青岛266021

出　　处：《中国食物与营养》2017年第3期54-58,共5页Food and Nutrition in China

摘　　要：目的:探讨孕期高果糖摄入对胚胎发育的影响及胎盘血管因子对其影响的机制。方法:成年雌性SD大鼠与正常饮食雄性大鼠进行交配,孕鼠随机分为5组,分别是对照组、正常剂量果糖组、高剂量果糖组、高剂量蔗糖组和超高剂量果糖组,在孕期分别给予1m L去离子水、1.6g/kg、4.8g/kg、4.5g/kg和8.0g/kg的果糖水和蔗糖水灌胃,连续干预3w,于第3w末处死孕鼠,麻醉、取血并剖取胎鼠,检查胎鼠的一般状况、着床、死胎、吸收胎、外观畸形等,制作子代内脏和骨骼标本,观察内脏和骨骼的情况;称量胎重和胎盘重,观察各组间胎盘血管内皮生长因子(VEGF)、血管内皮生长因子受体-1(s Flt-1)和一氧化氮(NO)的变化。结果:对孕鼠连续进行3w的果糖干预后,超高剂量果糖组子代出生体重显著低于对照组(P<0.05);高剂量果糖组、高剂量蔗糖组和超高剂量果糖组子代与对照组和正常剂量果糖组相比,死胎数和吸收胎数显著增加(P<0.05);但各组子代并没有发生骨骼畸形和内脏出血。超高剂量果糖组胎盘重量显著低于对照组(P<0.05);高剂量果糖组、高剂量蔗糖组和超高剂量果糖组胎盘中VEGF和NO水平显著低于对照组和正常剂量果糖组(P<0.05);胎盘中s Flt-1水平明显高于对照组和正常剂量果糖组(P<0.05)。结论:孕期高果糖摄入可增加子代发生死胎和吸收胎等不良结局的风险,胎盘中VEGF、NO的水平降低和s Flt-1水平增高可能是导致子代发生不良结局的原因,具体机制值得深入研究。[Objective] To study effects of high fructose consumption during pregnancy on fetal development and preliminarily study its mechanism. [Method] We examined the effects of feeding the pregnant mice with high fructose throughout pregnancy. Rats were randomly divided into control group, normal dose of fructose group, high dose fructose group, high dose sucrose group and super high dose fructose group. Through intragastrie administrations give different dosage of fructose to five groups. The test period was 3 weeks. Dams and offspring were killed in the third weekend anesthesia, bloodand embryo was checked about general situation of fetal rat including nidation number, number of stillbirth, absorbing birth and exterior deformation number and so on. We observed the internal organs and bones by making fetal rat organ and bone specimen. Weighing fetal weight and placental weight and to observe the changes of placental vascular endothelial growth factor （ VEGF）, solublefms-like tyrosine kinase-1 （sFlt-1） and nitric oxide （NO） . [Result] With the increase of dosage of fructose for pregnant rat, the preg- nant rats weight of high fructose group, high sucrose group and super high fructose group was significantly higher than control group （ P 〈 0. 05）, The fasting blood-glucose （FBG） was higher than control group and normal fructose group （P 〈0. 05） . the fetal weight of super high fructose group was lower than control group, normal fructose group, high fructose group and high sucrose group （P 〈0. 05） . Group, the num- ber of absorbing birth and stillbirth of high fructose group, high sucrose group and super high fructose group was significantly higher than control group and normal fructose group （P 〈0. 05）, but fructose feeding had no effect on the rate of skeletal deformities and visceral hemorrhage. The placental weight of super high fructose group was lower than control group （P 〈0. 05） . The placental lever of VEGF and NO of high fructose group, high sucrose group and super

关 键 词：果糖 子代 可溶性血管内皮生长因子受体-1 血管内皮生长因子 一氧化氮 

分 类 号：R153.1[医药卫生—营养与食品卫生学]