Bub1基因对人肝癌MHCC97-H细胞增殖、周期和凋亡的影响  

Influence of Bub1 Gene on the Cell Proliferation,Cell Cycle and Apoptosis in Human Hepatocellular Carcinoma Cell Line MHCC97-H

作　　者：蒲猛 汪建林[1] 黄启科[1] 赵戈[1] 夏聪聪[1] 何大立 杨针 陶开山[1] PU Meng WANG Jian-lin HUANG Qi-ke ZHAO Ge XIA Cong-cong HE Da-li YANG Zhen TAO KM-shan(Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Mech＇cal University, Xi＇nn, Shaanxi, 710032, China)

机构地区：[1]第四军医大学西京医院肝胆胰脾外科,陕西西安710032

出　　处：《现代生物医学进展》2017年第12期2201-2204,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81670593;81272648)

摘　　要：目的:研究Bub1基因在肝癌中的表达以及对肝癌细胞系MHCC97-H增殖、周期和凋亡的影响。方法:利用RNA干扰技术下调肝癌细胞系MHCC97-H中Bub1的表达;qRT-PCR和Western Blot分别检测Bub1在mRNA和蛋白水平表达的变化;CCK-8实验检测肿瘤细胞增殖能力的改变;流式细胞术检测细胞周期和凋亡的变化。结果:qRT-PCR和Western Blot结果显示si-Bub1能够成功下调Bub1的表达;下调Bub1后肝癌MHCC97-H细胞的增殖能力下降(P<0.05),细胞的凋亡比例升高(P<0.05),细胞发生S期阻滞。结论:Bub1基因在肝癌中高表达,下调Bub1的表达后能够降低肝癌细胞的增殖能力,促进细胞凋亡,诱导细胞发生S期阻滞。Objective： To study the expression of Bubl in hepatocellular carcinoma （HCC） and its effect on the cell proliferation, cell cycle and apoptosis in hepatocellular carcinoma cell line MHCC97-H. Methods： Bubl was down-regulated by small interfering RNA （siRNA） in MHCC97-H. qRT-PCR and Western blot were used to determine the expression of mRNA and protein. CCK-8 assay was performed to evaluate the proliferation ability. Apoptosis and cell cycles were determined by flow cytometry. Rosults： qRT-PCR and Western blot showed that si-Bubl group displayed a low expression ofBubl. Down-regulated of Bubl by siRNA significantly inhibited the cell proliferation （P〈0.05）, Cell cycle had changed and increased the cell apoptosis rates （P〈0.05）. Conclusion： Bubl was highly expressed in hepatocellular carcinoma and down-regnlating the expression of Bubl couldreduce the proliferation of hepatocellular carcinoma cells and affect the cell cycle and apoptosis of HCC.

关 键 词：肝癌 BUB1 增殖 细胞周期 细胞凋亡 

分 类 号：R-33[医药卫生] R735.7