植物乳杆菌35对大肠杆菌在肠上皮细胞HT-29上黏附及刺激产生促炎细胞因子的影响  

Effect of Lactobacillus plantarum 35 on Escherichia coli-induced IL-8 Production and E. coli Adhesion on HT-29 Cells

作　　者：郭远骥 妥彦峰[1] 牟光庆[1,2] 蒋淑娟[1] 钱方[1] 刘鹏[1] 盛晓芳[1] GUO Yuan-ji TUO Yan-feng MOU Guang-qing JIANG Shu-j uanl QIAN Fangl LIU Pengl SHEGN Xiao-fang(School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China The Innovation Center of Food Safety and Nutrition of Northeast Agricultural University, Harbin 150030, China)

机构地区：[1]大连工业大学食品学院,辽宁大连116034 [2]东北农业大学食品安全与营养协同创新中心,黑龙江哈尔滨150030

出　　处：《现代食品科技》2017年第3期14-21,共8页Modern Food Science and Technology

基　　金：国家十二五科技支撑计划项目(2013BAD18B09);国家自然科学基金项目(31571813);国家海洋食品工程技术研究中心项目(2012FU125X03);辽宁省自然科学基金项目(2014026018)资助

摘　　要：本论文采用肠上皮细胞模型HT-29细胞系研究8株植物乳杆菌在HT-29细胞上的黏附性,并研究黏附性较强的菌株及其胞外多糖(Exopoly Saccharides,EPS)抑制大肠杆菌(E.coli ATCC25922)在HT-29细胞上黏附及刺激HT-29细胞产生炎性因子的作用。结果表明8株植物乳杆菌在HT-29细胞上的黏附性差异较大:黏附性最强的植物乳杆菌35通过取代、竞争和排阻方式抑制大肠杆菌在HT-29细胞上黏附,抑制黏附率分别为30%、33%和59%,其EPS在作用浓度为500μg/mL时对大肠杆菌的抑制黏附率为32%。植物乳杆菌35可抑制大肠杆菌刺激HT-29细胞产生IL-8,通过取代、竞争、排阻方式抑制大肠杆菌刺激HT-29细胞产生IL-8,抑制率分别为3%、28%和40%;其EPS抑制大肠杆菌刺激HT-29细胞产生IL-8具有浓度效应,浓度为500μg/mL时抑制率最高,为50%。而对于IL-10表达量的影响均不显著。结果表明植物乳杆菌35具有抑制大肠杆菌引起肠炎的潜在益生功能。The adhesive ability of eight strains of Lactobacillus plantarum on the HT-29 intestinal epithelial cell line, and the inhibitory effect of the strain with the highest adhesive ability and its exopolysaccharides（EPS） on Escherichia coli-induced IL-8 production and E. coliadhesion on HT-29 cells were investigated. The results indicated that the eight Lactobacillus strains adhered to HT-29 cells to different degrees, and L. plantarum 35 had the highest adhesive ability. L. plantarum 35 could protect HT-29 cells from E. coli adhesion by displacement, competition, and exclusion, with inhibition rates of 30%, 33%, and 59%, respectively. At an active concentration of 500 μg/mL, EPS could inhibit E. coli adhesion by 32%. L. plantarum 35 could inhibit E. coli-induced IL-8 production by HT-29 cells by displacement, competition, and exclusion with inhibition rates of 3%, 28%, and 40%, respectively. EPS could inhibit E. coli-induced IL-8 production by HT-29 cells in a dose dependent manner, with the highest inhibitory rate of 50% at 500 μg/mL concentration. L. plantarum 35 had no effect on IL-10 production by HT-29 cells. The results showed that L. plantarum 35 has a potential probiotic function and may inhibit inflammatory bowel disease induced by E. coli.

关 键 词：乳酸杆菌 HT-29 大肠杆菌 黏附 

分 类 号：TS201.6[轻工技术与工程—食品科学]