低能氮离子注入和紫外线复合诱变选育高产酿酒酵母  

Mutation breeding of ethanol-producing yeast Saccharomyces cerevisiae by low-energy nitrogen ion implantation and UV rays

作　　者：虞龙[1] 姚驰亚 吴晓菲[1] 李玉燕[1] 张娣[1] 虞沐尧 YU Long YAO Chiya WU Xiaofei LI Yuyan ZHANG Di YU Muyao(College of Bioteehnology and Pharmaceutical Engineering, Nanjing University of technology, Nanjing 210000, China)

机构地区：[1]南京工业大学生物与制药工程学院,南京210000

出　　处：《辐射研究与辐射工艺学报》2017年第2期44-49,共6页Journal of Radiation Research and Radiation Processing

基　　金：国家高技术研究发展计划(863计划)(2012AA023400)~~

摘　　要：通过离子注入联合紫外线辐射诱变选育高产酿酒酵母菌株,根据存活率及突变率确定离子注入的诱变条件为电荷18 ke V,剂量400×10^(14) cm^(-2),紫外线辐射60 s。在该条件下,诱变获得的菌株正突变率和致死率都最高,诱变效果最好。在该条件下进行研究后获得目标菌株SC-N9015,酒精产量从14.65%提升到15.53%。多次传代实验结果显示遗传性状稳定。研究表明,通过低能氮离子注入和紫外线联合诱变选育高产酒精酵母效果显著。The ethanol-producing yeast Saccharomyces cerevisiae was induced by N^＋ implantation with an output power of 18 keV and a dose of 400×10^14cm^-2,depending on the survival and mutation rates. The UV exposure time was 60 s. Under optimized conditions, the strain achieved the highest positive mutation and lethality rates. A mutant strain SC-N9015 was selected; the ethanol yield of this strain was 15.53%, whereas that of the parental strain was 14.65%. Further, evaluation of ethanol yield over successive generations indicated the hereditary stability of the mutations. The study shows that the combination of low energy N＋implantation with UV rays is an efficient method for mutation breeding of S. cerevisiae.

关 键 词：酿酒酵母 低能氮离子注入 紫外线 辐射 诱变筛选 

分 类 号：TL99[核科学技术—核技术及应用] Q691.5[生物学—生物物理学]