UPLC-MS／MS技术测定猪肉中甲氧氯普胺不确定度评价  

Uncertainty evaluation for the determination of metoclopramide residue in pork by UPLC-MS/MS

作　　者：曹慧[1] 施元旭[1] 王璐璐[1] 

机构地区：[1]浙江省产品质量安全检测研究院浙江省食品安全重点实验室,浙江杭州310018

出　　处：《食品工业科技》2017年第9期267-270,共4页Science and Technology of Food Industry

基　　金：浙江省科技厅公益性科技计划(2015C37019);浙江省质量技术监督系统科研计划(20150204)资助项目

摘　　要：对超高效液相色谱-串联质谱测定猪肉中甲氧氯普胺残留量过程中可能影响到结果准确性的因素进行了分析。根据行业标准SN/T 2227-2008《进出口动物源性食品中甲氧氯普胺残留量检测方法液相色谱法-质谱/质谱法》,建立不确定度评价的数学模型,通过不确定度来源分析建立不确定度的评价方法。结果表明:标准溶液浓度产生的不确定最大,随机效应引入的不确定度次之,样品定容产生的不确定度最小。猪肉中甲氧氯普胺残留量为50.1μg/kg时,其扩展不确定度为5.0μg/kg(k=2)。An uncertainty evaluation was established for evaluating the determination results of metoclopramide in pork by UPLC-MS/MS.According to the determination of naetoclopramide residue in foodstuffs of animal origin for import and export- LC- MS/MS method （ SN/T 2227-2008 ） , the mathematical model of uncertainty was established and the sources of uncertainty were analyzed.As a result, the concentration of standard solution was the biggest factor for the uncertainty in measurement, the second was the repetition, the volume of sample was the smallest factor.The residue of metoclopramide in pork was 50.1 μg/kg, the expanded uncertainty was 5.0 μg/kg（ k = 2）.

关 键 词：不确定度 甲氧氯普胺 超高效液相色谱-串联质谱 猪肉 

分 类 号：TS251.1[轻工技术与工程—农产品加工及贮藏工程]