柚皮苷对脑缺血再灌注损伤的保护作用  

The protective effect of naringin on cerebral ischemia reperfusion injury

作　　者：刘微[1] 张洋[1] 郭建超[1] 练鹏影 吴敏华[1] 丁文婷[1] 

机构地区：[1]广州中医药大学基础医学院,广东广州510006

出　　处：《中风与神经疾病杂志》2017年第4期292-294,共3页Journal of Apoplexy and Nervous Diseases

基　　金：国家自然科学基金资助项目(No.81202817;81673772);广东省高等学校优秀青年教师培养项目(No.Yq2013041);高等学校博士学科点专项科研基金(No.20124425120011)

摘　　要：目的研究柚皮苷(Naringin,NRG)对大鼠局灶性脑缺血再灌注损伤的保护作用及星形胶质细胞是否参与其中。方法柚皮苷连续灌胃7 d后进行大脑中动脉栓塞(middle cerebral artery occlusion,MCAO),缺血2 h后拔出线栓进行再灌注。24 h后TUNEL染色观察凋亡细胞数量,苏木精-伊红(hematoxyli-eosin,HE)染色观察形态学改变,免疫荧光染色分别检测星形胶质细胞标记物胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)和缝隙连接蛋白43(connexin43,Cx43)。结果同模型组相比,柚皮苷预干预后凋亡细胞数量显著减少(P<0.05),细胞损伤明显减轻。免疫染色显示柚皮苷可以减轻星形胶质细胞的激活程度,同时Cx43的表达也有所降低(P<0.05)。结论柚皮苷预干预可有效减轻脑缺血再灌注损伤,该保护作用同降低星形胶质细胞Cx43表达密切相关。Objective To study the protective effects of naringin on rats with focal cerebral ischemia and reperfusion and whether astroeyte participates in this effect. Methods Naringin group was pretreated intragastricly for consecutive 7 days before ischemia. Middle cerebral artery was occluded for 2 h and then reperfused for 24 h. The number of apoptotic cells was detected by TUNEL staining, and pathological changes were observed by hematoxyli-eosin （ HE ） staining, hnmunofluorescent staining was used to observe astrocytic marker （ glial fibrillary detection acidic protein, GFAP） and connexin43 （Cx43）. Results Compared with isehemia group, pretreatment with naringin reduced the number of apoptotic cells （ P 〈 0.05 ） , and significantly attenuated cell injury. Immunofluorescent staining showed that naringin attenuated astrocytic activation, and decreased the expression of Cx43 （P 〈 0.05 ）. Conclusion Naringin pretreatment can effectively attenuated cerebral ischemia reperfusion injury, and the protective effects are closely related with Cx43 in astrocytes.

关 键 词：柚皮苷 缺血再灌注 星形胶质细胞 CX43 

分 类 号：R743.3[医药卫生—神经病学与精神病学]