HPLC法测定莲子种皮中没食子酸的含量  

Determination content of gallic acid in lotus seed coat by HPLC

作　　者：潘国庆[1,2] 黄敏[1] 夏千秋 钱宇超[1] 

机构地区：[1]青海民族大学药学院,西宁810007 [2]青海省植物化学资源重点实验室,西宁810007

出　　处：《食品科技》2017年第4期293-297,共5页Food Science and Technology

摘　　要：目的:测定莲子种皮中没食子酸的含量。方法:通过单因素实验考察乙醇浓度、料液比、超声时间3个因素对酚性物质提取率的影响。通过正交实验分析,最佳提取工艺条件为:乙醇浓度50%,料液比1:10,超声时间10 min。没食子酸含量测定采用高效液相色谱法(HPLC),其流动相为甲醇-0.2%磷酸(5:95),检测波长273 nm,流速0.8 mL/min。采用外标一点法测定莲子种皮中没食子酸的含量。结果:没食子酸在(9.12~82.08)μg范围内呈良好的线性关系(r=0.9998),平均回收率为98.46%,RSD为1.22%(n=9)。在该实验条件下,测得莲子种皮中没食子酸的含量为2.7513 mg/g。结论:该实验方法简单快速,重现性好,可为莲子种皮的综合利用提供科学依据。Objective： To determine the content of gallic acid in lotus seed coat. Methods： Through single factor experiment to study the ethanol concentration, solid-liquid ratio, ultrasonic time three factors on the extraction rate of phenolic substances. Through orthogonal experiment, the optimum extraction conditions were as follows： ethanol concentration 50%, solid-liquid ratio 1：10, ultrasonic time 10 min. The content of gallic acid was determined by high performance liquid chromatography （HPLC）, the mobile phase consisted of methanol -0.2% phosphoric acid （5：95）. The detection wavelength was 273 nm and the flow rate was 0.8 mL/min. The content of gallic acid in lotus seed coat was determined by external standard method. Results： The calibration curve showed good linearity within the range of （9.12-82.08）pg. The average recovery was 98.47% and RSD was 1.22% （n=9）. Under the experimental conditions, the measured gallic acid of lotus seed coat was 2.7513 mg/g. Conclusion： The experimental method was simple, rapid and reproducible, and could provide scientific basis for comprehensive utilization of lotus seed coat.

关 键 词：没食子酸 莲子种皮 高效液相色谱法 含量 

分 类 号：TS207.3[轻工技术与工程—食品科学]