Morris水迷宫训练对老年痴呆大鼠模型学习记忆能力及海马组织肿瘤坏死因子-α、超氧化物歧化酶、丙二醛水平的影响  

Influence of Morris water maze training on Azheimer's disease rats learning and memory ability and the expressions of TNF-α,SOD and MDA in hippocampal tissue

作　　者：彭璇[1] 吴刚[2] 吴昊[1] 黄德斌[1] 

机构地区：[1]湖北民族学院医学院,湖北恩施445000 [2]湖北民族学院医学院病理解剖学教研室

出　　处：《中国老年学杂志》2017年第8期1833-1835,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(No.81360654)

摘　　要：目的观察Morris水迷宫训练对老年痴呆(AD)大鼠学习记忆能力及海马组织肿瘤坏死因子(TNF)-α、超氧化物歧化酶(SOD)、丙二醛(MDA)的影响。方法随机将32只SD雄性大鼠分为假手术组、模型组、Morris水迷宫短期训练组和Morris水迷宫长期训练组;以穹隆-海马伞切断建立痴呆大鼠模型;运用Morris水迷宫进行训练;采用跳台实验以及水迷宫实验测试大鼠的学习和记忆能力;采用酶联免疫吸附法(ELISA)测定大鼠海马组织TNF-α的水平,比色法检测大鼠海马组织SOD活性和MDA含量。结果 Morris水迷宫训练后AD大鼠学习记忆能力显著提高(P<0.01);大鼠海马区TNF-α、MDA含量明显降低(P<0.05)、SOD含量明显升高(P<0.05)。结论 Morris水迷宫训练能明显改善大鼠的学习记忆能力,其机制可能与提高海马区SOD含量、降低TNF-α和MDA水平有关,而且水迷宫训练时间越长,恢复越明显。Objective To investigate the protective effect of water maze training on Alzheimer,s disease（AD）rats.Methods 32 SD rats were randomly divided into sham,model,short-term Morris water-maze training（ short-term） and long-term Morris water-maze training（ long-term） groups.Rat model of AD was made by transection of rat fimbria-fornix. Rats in these groups were tested for spatial learning and memory ability in Step-down Test and Morris water maze.Then,the brains were removed and hippocampal homogenate were prepared.Enzyme linked immunosorbent assay（ ELISA） was adopted to measure the level of TNF-α,colorimetric method was used to detect the content of superoxide dismutase（ SOD） and malondialdehyde（ MDA） in the hippocampus homogenate. Results Morris water-maze training,learning and memory ability of AD rat was increased obviously,TNF-α and MDA were reduced,SOD was increased in hippocampal homogenate（ P〈0. 05）.Conclusions Water maze training can improve the spatial learning and memory ability of AD rats made by transaction of fimbria-fornix by enhancing the content of SOD and reducing TNF-α and MDA levels,and longer training and better effect.

关 键 词：Morris水迷宫训练 痴呆 学习和记忆能力 大脑海马 

分 类 号：R73-3[医药卫生—肿瘤]