基于旋转设计优化胶原蛋白ACE抑制肽制备工艺  

Enzyme Hydrolysis Preparation Model of Collagen-derived ACE Inhibitory Peptides Based on Quadratic General Rotation Design

作　　者：于志鹏[1] 张霜[1] 赵文竹[1] 张倩[1] 沈俊彤 王瑜[1] 霍倩倩[1] 励建荣[1] 刘静波[2] YU Zhi-peng ZHANG Shuang ZHAO Wen-zhu ZHANG Qian SHEN Jun-tong WANG Yu HUO Qian-qian LI Jian-rong LIU Jing-bo(College of Food Science and Engineering, Bohai University, National ＆ Local Joint Engineering Research Center of Storage Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Jinzhou 121013, Liaoning, China Lab of Nutrition and Functional Food, Jilin University, Changehun 130062, Jilin , China)

机构地区：[1]渤海大学食品科学与工程学院,生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心,辽宁锦州121013 [2]吉林大学营养与功能食品研究室,吉林长春130062

出　　处：《食品研究与开发》2017年第7期59-63,共5页Food Research and Development

基　　金：国家科技支撑课题(2012BAD00B03);辽宁省科学事业公益研究基金项目(2016004004);渤海大学博士启动项目(0515bs079);辽宁省科技攻关项目(2015103020)

摘　　要：采用Protamex~蛋白酶水解鱼胶原蛋白源制备血管紧张素转化酶(ACE)抑制肽,并采用p H-stat法测定水解度,对影响水解效果的p H值、温度、底物浓度和加酶量4个因素进行考察,并进行三元二次通用旋转设计进行优化,对活性肽的ACE抑制活性和结构进行分析。结果表明在底物浓度11.8%,p H 8.7,加酶量5.4%和酶解温度53.6℃条件下,酶解1 h所得的鱼皮胶原蛋白水解度为16.6%,经高效液相测定鱼皮ACE抑制活性为45%,经圆二色谱分析鱼胶原蛋白ACE抑制肽主要以无规卷曲形式存在。Fish-derived collagen was hydrolyzed by protamex R protease and measured by the degree of hydrol-ysis. The hydrolysis model of peptides from fish-derived collagen was established through quadratic general ro-tation design followed single factor experiment, subsequently ACE inhibitory activity and secondary structure of fish collagen-derived peptides was measured. The results showed that the equation for the optimal regression e-quation had a maximal value according to regression coefficient test, regression equation, and the lack of fit test. The optimal details of enzymatic hydrolysis was followed：substrate concentration 11.8%, enzymatic hy-drolysis pH value 8.7, the amount of enzyme 5.4%and hydrolysis temperature 53.6℃, and hydrolysis degree of fish-derived collagen was 16.6 % in one hour. In vitro ACE inhibitory activity of bioactive peptides from fish-derived collagen was performed by high performance liquid chromatography, and the value of activity a-gainst ACE was 45 %. In addition, secondary structure of fish collagen-derived ACE inhibitory peptide was random coil.

关 键 词：鱼胶原蛋白 血管紧张素转化酶 活性肽 二级结构 

分 类 号：TQ464.7[化学工程—制药化工]