Neuroprotective mechanism of Kai Xin San: upregulation of hippocampal insulin-degrading enzyme protein expression and acceleration of amyloid-beta degradation  

作　　者：Na Wang Yong-ming Jia Bo Zhang Di Xue Maharjan Reeju Yan Li Shu-ming Huang Xue-wei Liu 

机构地区：[1]Institute of Medicine,Qiqihar Medical University [2]Department of Neuroscience,Institute of Traditional Chinese Medicine,Heilongjiang University of Chinese Medicine

出　　处：《Neural Regeneration Research》2017年第4期654-659,共6页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,No.81303248,81603321;the Natural Science Foundation of Heilongjiang Province of China,No.H2015028;a grant from the Nursing Program for Young Scholars of Heilongjiang Province of China,No.UNPYSCT-2016116;the Scientific Research Fund for Doctors of Qiqihar Medical University in China,No.QY2016B-09

摘　　要：Kai Xin San is a Chinese herbal formula composed of Radix Ginseng, Poria, Radix Polygalae and Acorus Tatarinowii Rhizome. It has been used in China for many years for treating amnesia. Kai Xin San ameliorates amyloid-β （Aβ） induced cognitive dysfunction and is neuroprotective in vivo, but its precise mechanism remains unclear. Expression of insulin-degrading enzyme （IDE）, which degrades Aβ, is strongly correlated with cognitive function. Here, we injected rats with exogenous Aβ42 （200 μM, 5 μL） into the hippocampus and subsequently administered Kai Xin San （0.54 or 1.08 g/kg/d） intragastrically for 21 consecutive days. Hematoxylin eosin and Nissl staining revealed that Kai Xin San protected neurons against Aβ-induced damage. Furthermore, enzyme linked immunosorbent assay, western blot and polymerase chain reaction results showed that Kai Xin San decreased Aβ42 protein levels and increased expression of IDE protein, but not mRNA, in the hippocampus. Our findings reveal that Kai Xin San facilitates hippocampal Aβ degradation and increases IDE expression, which leads, at least in part, to the alleviation of hippocampal neuron injury in rats.Kai Xin San is a Chinese herbal formula composed of Radix Ginseng, Poria, Radix Polygalae and Acorus Tatarinowii Rhizome. It has been used in China for many years for treating amnesia. Kai Xin San ameliorates amyloid-β （Aβ） induced cognitive dysfunction and is neuroprotective in vivo, but its precise mechanism remains unclear. Expression of insulin-degrading enzyme （IDE）, which degrades Aβ, is strongly correlated with cognitive function. Here, we injected rats with exogenous Aβ42 （200 μM, 5 μL） into the hippocampus and subsequently administered Kai Xin San （0.54 or 1.08 g/kg/d） intragastrically for 21 consecutive days. Hematoxylin eosin and Nissl staining revealed that Kai Xin San protected neurons against Aβ-induced damage. Furthermore, enzyme linked immunosorbent assay, western blot and polymerase chain reaction results showed that Kai Xin San decreased Aβ42 protein levels and increased expression of IDE protein, but not mRNA, in the hippocampus. Our findings reveal that Kai Xin San facilitates hippocampal Aβ degradation and increases IDE expression, which leads, at least in part, to the alleviation of hippocampal neuron injury in rats.

关 键 词：nerve regeneration NEURODEGENERATION traditional Chinese medicine Kai Xin San insulin-degrading enzyme amyloid-β Alzheimer＇sdisease Chinese herbal compound Aβ-degrading enzymes neurons Radix Ginseng Radix Polygalae Acorus Tatarinowii Rhizoma neuralregeneration 

分 类 号：R749.16[医药卫生—神经病学与精神病学]