小麦TaLox-B等位变异对脂肪氧化酶活性和面粉色泽的影响  

Effects of TaLox-B Alleles on Lipoxygenase Activity and Flour Color in Wheats

作　　者：张福彦[1] 陈锋[2] 程仲杰[1] 杨保安[1] 范家霖[1] 陈晓杰[1] 张建伟[1] 陈云堂[1] 崔龙[1] 

机构地区：[1]河南省科学院同位素研究所有限责任公司/河南省核农学重点实验室,郑州450015 [2]河南农业大学农学院/河南省粮食作物协同创新中心,郑州450002

出　　处：《中国农业科学》2017年第8期1370-1377,共8页Scientia Agricultura Sinica

基　　金：河南省基础与前沿技术研究计划(162300410169);河南省科技攻关计划(172102110069);河南省小麦产业技术体系建设专项资金(Z2010-01-04)

摘　　要：【目的】小麦籽粒脂肪氧化酶(Lox)与面粉色泽密切相关,研究TaLox-B位点上的不同等位变异类型对Lox活性和面粉色泽的影响,为面粉色泽的改良和相关的品质育种工作提供参考依据。【方法】选用122份河南小麦品种(系)为试验材料,用紫外分光光度计和色差仪分别测定其Lox活性和面粉色泽,并利用控制脂肪氧化酶活性位于4BS上的TaLox-B1、TaLox-B2和TaLox-B3位点上的功能标记Lox16、Lox18和Lox-B23对参试材料的Lox基因型进行鉴定。【结果】不同品种间Lox活性及其色泽性状差异达到极显著水平。基因型鉴定结果表明,参试材料的TaLox-B1位点存在TaLox-B1a和TaLox-B1b两种等位变异,所占比例分别为63.9%和39.1%,TaLox-B2位点存在TaLox-B2a和TaLox-B2b两种等位变异,所占比例分别为57.4%和42.6%,TaLox-B3位点也存在TaLox-B3a和TaLox-B3b两种等位变异,所占比例分别为41.8%和48.2%。分析其基因型组合发现,参试材料中共有6种基因型组合类型,依次为TaLox-B1a/TaLox-B2a/TaLox-B3a、TaLox-B1a/TaLox-B2a/TaLox-B3b、TaLox-B1a/TaLox-B2b/TaLox-B3b、TaLox-B1b/TaLox-B2a/TaLox-B3a、TaLox-B1b/TaLox-B2a/TaLox-B3b和TaLox-B1b/TaLox-B2b/TaLox-B3b,所占比例分别为41.8%、15.6%、6.6%、28.7%、5.7%和1.6%。分析不同TaLox-B位点基因与Lox活性及红度(a*值)、黄度(b*值)、亮度(L*值)、白度(Wht值)等面粉色泽性状的关系表明,单基因等位变异对Lox活性和面粉色泽的影响不同,3个基因等位变异a*值差异均不显著,对Lox活性的效应,TaLox-B2a高于TaLox-B2b(P<0.05),TaLox-B3a高于TaLox-B3b(P<0.01),TaLox-B2a基因型的Wht值低于TaLox-B2b基因型(P<0.01),TaLox-B3a基因型的Wht值也低于TaLox-B3b基因型(P<0.05)。说明TaLox-B2和TaLox-B3对Lox活性和面粉色泽具有重要影响。进一步分析发现,拥有TaLox-B1a/TaLox-B2a/TaLox-B3a基因型组合的小麦品种(系)的Lox活性和b*值最高,a*值和Wht值最低,而TaLox-B1b/TaLox-B2b/TaLox-B3b基因型组合的小麦品种【Objective】Lipoxygenase（Lox） is closely related to flour color.The objective of this study was to investigate the influence of different allelic variations at TaLox-B loci on Lox activity and flour color in wheat to provide a theoretical basis for improving flour color and related quality traits in breeding programs.【Method】A total of 122 wheat cultivars and advanced lines from Henan province were used to measure the Lox activities and flour color by spectrophotometer and colorimeter.The functional markers Lox16,Lox18 and Lox-B23 were used to identify the polymorphism of the Lox genes on chromosome 4BS in current Henan wheat cultivars and advanced lines.【Result】There were significant differences of Lox activities,L＊,a＊ and b＊values as well as Wht data among different cultivars.Genotyping results indicated two alleles TaLox-B1 a and TaLox-B1 b at TaLox-B1 locus in these wheat varieties,with 63.9% and 39.1%,respectively.At TaLox-B2 locus,two alleles TaLox-B2 a and TaLox-B2 b were identified by the functional marker Lox-B23,with 57.4% and 42.6%,respectively.It was also found two alleles TaLox-B3 a and TaLox-B3 b at the TaLox-B3 locus,with 41.8% and 48.2%,respectively.Six allele combinations of Lox genes,i.e.,TaLox-B1a/TaLox-B2a/TaLox-B3 a,TaLox-B1a/TaLox-B2a/TaLox-B3 b,TaLox-B1a/TaLox-B2b/TaLox-B3 b,TaLox-B1b/TaLox-B2a/TaLox-B3 a,TaLox-B1b/TaLox-B2a/TaLox-B3 b and TaLox-B1b/TaLox-B2b/TaLox-B3 b were found with 41.8%,15.6%,6.6%,28.7%,5.7% and 1.6%,respectively.Analysis of association of Lox alleles at different TaLox-B loci and Lox activities and flour color parameters indicated that the allelic variation at a single locus showed diverse effects on Lox activities and flour color.For a＊ value,the allelic effect was not significant.For Lox activities,the order of effect was TaLox-B2 a TaLox-B2b（P0.05）,TaLox-B3 aTaLox-B3b（P0.01）.The Wht value of TaLox-B2 a was significantly lower than that of TaLox-B2b（P0.01）,and TaLox-B3 a showed also a significant lower Wht value than TaLox-B3b（P0

关 键 词：普通小麦 脂肪氧化酶基因 功能标记 面粉色泽 河南 

分 类 号：TS211[轻工技术与工程—粮食、油脂及植物蛋白工程] S512.1[轻工技术与工程—食品科学与工程]