九节菖蒲中酚酸类成分的提取工艺研究  

Study on the Extraction Technology of Phenolic Acids from Anemone altica

作　　者：杨晓东[1] 李福兵[1] 梅娇[1] 袁宏佳[1] 刘兴文[1] 李波[1] 

机构地区：[1]重庆市北碚区中医院药学部,重庆400700

出　　处：《中国药房》2017年第13期1848-1851,共4页China Pharmacy

摘　　要：目的:测定九节菖蒲中酚酸类成分含量并优化其提取工艺。方法:采用高效液相色谱法测定九节菖蒲中单阿魏酰酒石酸、阿魏酸的含量;以2种指标性成分的总含量为指标,以提取溶剂的体积分数、提取溶剂量、提取次数和提取时间为因素,采用正交试验法优化提取工艺,并进行验证试验。结果:九节菖蒲药材中单阿魏酰酒石酸和阿魏酸的含量分别为0.059%、0.002 5%。优化后的提取工艺为将30%乙醇600 mL加至20 g药材中提取2次,每次90 min;验证试验中2种成分在提取物中的平均含量分别为0.297 1%(RSD=3.64%,n=3)、0.004 1%(RSD=5.11%,n=3)。结论:建立了九节菖蒲中单阿魏酰酒石酸、阿魏酸含量的测定方法;优化后的提取工艺稳定、可行。OBJECTIVE：To determine the content of phenolic acids from Anemone altica,and optimize its extraction technolo-gy. METHODS：HPLC was used to determine the contents of mono-ferulyl tartaric acid and ferulic acid from A. altica;using the total contents of 2 index components as index,volume fraction of extraction solvent,extraction solvent volume,extraction times and extraction time as factors,orthogonal test was used to optimize extraction technology,and verification test was conducted. RE-SULTS：The contents of mono-ferulyl tartaric acid and ferulic acid were 0.059%,0.0025%,respectively;the optimal extraction technology was as follow as 30% ethanol 600 mL added to 20 g medicinal material,extracted twice,90 min every time. In verifi-cation test,the average contents of 2 components in extract were 0.2971%（RSD=3.64%,n=3）,0.0041%（RSD=5.11%,n=3）. CONCLUSIONS：A method for contents determination of mono-ferulyl tartaric acid and ferulic acid from A. altica is estab-lished;optimized extraction technology is stable and feasible.

关 键 词：九节菖蒲 酚酸类成分 单阿魏酰酒石酸 阿魏酸 含量测定 提取工艺 高效液相色谱法 正交试验 

分 类 号：R284.2[医药卫生—中药学]