甘油和生物素对酿酒酵母产胞内谷氨酸的影响  

Effects of Glycerol and Biotin additions on intracellular Glutamate Production by Saccharomyces cerevisiae

作　　者：刘立聪[1] 汤超[1] 王志[1] 代俊[1] 李欣[1] 姚娟[2] 郑国斌[2] 吕正波 陈雄[1] LIU Licong TANG Chao WANG Zhi DAI Jun LI Xin YAO Juan ZHENG Guobin LYU Zhengbo CHEN Xiong(Key Laboratory Fermentation Engin. （Ministry of Education）, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, College of Food and Bioengin., Hubei Univ. of Tech., Wuhan 430068,China Hubei Provincial Key Laboratory of Yeast Function ,Angel Yeast Co., Ltd ,Yichang 443003,China Angel Yeast （De Hong） Co., Ltd, Dehong 678400.China)

机构地区：[1]湖北工业大学生物工程与食品学院、发酵工程教育部重点实验室、工业发酵湖北省协同创新中心,湖北武汉430068 [2]湖北省酵母功能重点实验室,安琪酵母股份有限公司,湖北宜昌443003 [3]安琪酵母(德宏)有限公司,云南德宏678400

出　　处：《湖北工业大学学报》2017年第2期42-46,共5页Journal of Hubei University of Technology

摘　　要：为降低Crabtree效应,提高酿酒酵母胞内谷氨酸的产量,研究补料物质分别为纯糖蜜、甘油加糖蜜以及甘油加糖蜜加生物素等3种条件下酿酒酵母的生长代谢。结果表明:用甘油替代部分糖蜜进行流加,可以降低Crabtree效应,但碳源利用率显著降低;在此基础上添加生物素后,既降低了Crabtree效应,又解决了碳源利用率低的问题;其中流加800mL糖蜜、300g甘油和4mg生物素时,胞内谷氨酸含量在12h达到3.04%,比只流加糖蜜时的峰值2.28%(18h)提高33.3%,而且发酵周期缩短了6h。In order to reduce the Crabtree effect and increase the intracellular glutamate production by Saccharomyces cerevisiae, the growth and metabolism of Saccharomyces cerevisiae has been studied under three conditions： pure molasses addition, molasses addition with glycerol, molasses addition plus glycerol with biotin. The results show that the molasses addition, partly substituted by glycerol, can reduce the Crabtree effect, but the carbon source utilization rate has significantly decreased. Molasses addition plus glycerol with biotin batch culture has reduced not only the Crabtree effect, but also resolved the problem of a low carbon source utilization rate by molasses addition with glycerol batch culture. When the fed batch culture was done with molasses （800 mL） as well as glycerol （300 g） and biotin （4 mg）, the intracellular glutamate content reached 3.04% at 12 h, which was 33.3% higher than that of the pure molasses 2.28% （18 h）, and the fermentation period was shortened by 6 h.

关 键 词：Crabtree效应 酿酒酵母 甘油 生物素 胞内谷氨酸 

分 类 号：Q815[生物学—生物工程]