肌醇六磷酸对二甲肼诱导的结直肠癌大鼠E-cadherin,TGF-β和VEGF表达的影响（英文）  

Inositol Hexaphosphate(IP6) Regulates the mRNA Expression of E-cadherin,TGF-β and VEGF in DMH-induced Colorectal Cancer Rat Model

作　　者：刘畅[1] 刘翠萍[1] 宋扬[1] 于文洋[1] 程立雪[1] 陈沉[1] 

机构地区：[1]青岛大学医学院营养所,山东青岛266021

出　　处：《现代生物医学进展》2017年第13期2414-2417,共4页Progress in Modern Biomedicine

基　　金：Natural Science Foundation of China(81373001)~~

摘　　要：目的:探索肌醇六磷酸(IP6)对结直肠癌大鼠E-钙黏蛋白(E-cadherin)、转化生长因子β(TGF-β)和血管生成因子(VEGF)表达的影响。方法:将60只Wistar大鼠分成五组分别为:空白组(CG)、DMH组(DG)、低剂量IP6组(LG)、中剂量IP6组(MG)、高剂量IP6组(HG)。LG、MG和HG大鼠每天分别以0.25 g/kg、0.5 g/kg、1.0 g/kg剂量的植酸钠灌胃,CG和DG大鼠以同体积的生理盐水灌胃。除去空白组的大鼠,其他四组大鼠每周进行一次颈背部皮下注射30 mg/kg的DMH诱导结直肠癌肿瘤,一共注射18周。注射18周后继续喂养16周到实验结束。运用实时定量PCR的方法检测E-cadherin、TGF-β和VEGF的表达。结果:DG组E-cadherin mRNA的表达明显低于其他各组(P<0.05),并且随着IP6浓度的增加E-cadherin的表达逐渐增加。相反,DG里TGF-β和VEGF的表达高于其他各组(P<0.05),且随着IP6浓度的增加TGF-β和VEGF的表达在减少。结论:IP6可以上调DMH诱导的结肠癌大鼠体内E-cadherin的表达和下调TGF-β、VEGF的表达。Objective：To explore the effect of Inositol Hexaphosphate （IP6） on expression of E-cadherin,TGF-β and VEGF in DMH-induced Colorectal Cancer （CRC） rat model.Methods：60 Wistar rats were assigned to 5 groups：Control group （CG）,DMH group （DG）,Low IP6 dose group （LG）,Moderate IP6 dose group （MG） and High IP6 dose group （HG）.The rats in LG,MG and HG were given sodium phytate with a dose of 0.25 g/kg,0.5 g/kg,1.0 g/kg for each group by gavage every day,respectively.Meanwhile,the rats in CG and DG were given the same volume of normal saline by gavage every day.The rats in DG,LG,MG,HG were subcutaneously injected with DMH （30 mg/kg） once a week for 18 weeks.The rats were fed for 16 weeks after the induced-tumor experiment.The mRNA expression levels of E-cadherin,TGF-β,VEGF were measured by real-time PCR.Results：The mRNA level expression of E-cadherin decreased obviously in DG （P〈0.05）.With the increase of IP6 concentration,the E-cadherin gradually increased.By contrast,the expression of TGF-β and VEGF was the highest in DG （P〈0.05） and both of them gradually decreased with increase ofIP6 concentration.Conclusions：IP6 could increase the mRNA level expression of E-cadherin in DMH-induced CRC rat model and decrease the expression of TGF-β and VEGF.

关 键 词：肌醇六磷酸 二甲肼 E-钙黏蛋白 转化生长因子β 血管内皮生成因子 Inositol Hexaphosphate (IP6) TGF-Β 

分 类 号：R735.3[医药卫生—肿瘤]