基于合成非病毒基因载体的教学实验开发  

Development of teaching experiments on synthesis of non-viral gene carriers

作　　者：夏玉琼[1] 谢晖[1] 曾琦[1] 程贺[1] 梁德海[2] 

机构地区：[1]西安电子科技大学生命科学技术学院,陕西西安710126 [2]北京大学化学与分子工程学院,北京100871

出　　处：《实验室科学》2017年第2期47-50,59,共4页Laboratory Science

基　　金：西安电子科技大学新实验开发与新实验设备及实验教学改革项目"聚乙烯亚胺/DNA复合物的Zeta电势和粒径分析"(项目编号:SY1465)

摘　　要：基因载体构建是生物制药技术课程中的重要内容,也是生物医药领域的前沿技术。常见的基因载体包括病毒载体和非病毒载体。非病毒载体能够有效地包载基因,操作安全简便,但是其转染效率远远不及病毒载体。通过改变非病毒基因载体的结构、尺寸、表面电荷等性质,人们能够有效地提高其转染效率。基于此,本实验设计了常用基因载体支化聚乙烯亚胺(bPEI)与DNA在不同电荷比的复合实验,并对复合物粒子的粒径和电势变化进行表征。该实验不仅可以让学生掌握粒径仪的工作原理和使用方法,通过对实验条件的摸索,还能够加深学生对非病毒基因载体的认识。The construction of gene carrier, a leading technology in biopharmaceuticals, plays an im- portant role in the course of ＂Pharmaceutical Biotechnology＂. Gene carriers include viral carriers and non-viral carriers. Non-viral gene carriers are safer and have no limitation on the loaded gene, but their transgenic efficiency is much lower than that of the viral counterpart. It has been demonstrated that the transgenic efficiency of non-viral carriers can be improved by adjusting their physicochemical properties, such as structure, size, and surface charge. Based on this, a simple experiment was de- signed to prepare the complex particles formed by branched PEI and DNA at different charge ratios, and to characterize the size and Zeta potential of the particles. This experiment not only allows students to know more about the gene carriers, but also help them to operate size analyzer, learn its working principles by optimization of experimental conditions.

关 键 词：基因载体 生物制药 粒径 Zeta电势 

分 类 号：Q782[生物学—分子生物学] G482[文化科学—教育学]