Frizzled 3、Frizzled 4基因对黑线仓鼠A:CHA白化性状产生影响的初步分析  

Analysis of the function of Frizzled 3、Frizzled 4 genes in the albino phenotype of Cricetulusgriseus

作　　者：王新[1] 刘慧芳[1,2] 易思萌 殷慧慧[3] 孙兆增[3] 

机构地区：[1]青岛农业大学,山东青岛266109 [2]临沂市农业学校,山东临沂276600 [3]军事医学科学院实验动物中心,北京100071

出　　处：《黑龙江畜牧兽医》2017年第5期222-224,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(31272387)

摘　　要：为了比较Wnt通路重要结合受体Frizzled家族的Frizzled 3、Frizzled 4受体蛋白基因在黑线仓鼠及其白化突变系(A:CHA)皮肤组织的表达差异,分析这两种基因对白化性状产生的影响,试验提取黑线仓鼠和A:CHA的皮肤组织总RNA,利用普通PCR方法筛选引物,通过实时荧光定量PCR技术比较黑线仓鼠和A:CHA皮肤组织Frizzled 3、Frizzled 4基因表达量的差异。结果表明:黑线仓鼠皮肤中的Frizzled 3、Frizzled 4基因mRNA的相对表达量分别是A:CHA组织中的4倍、5倍。Frizzled 3、Frizzled 4基因在A:CHA皮肤组织中的表达量下调。说明Frizzled 3、Frizzled 4基因与A:CHA白化性状的产生存在一定关联。In the current study, investigation and analysis of the expression level of Frizzled 3, Frizzled 4 of animportantreceptor of Wnt protein between Cricetulusgriseus and A ： CHA and the funetionon the albino phenotype were carried out. The total mRNA of skin tissue in the Cricetulusgriseus and A ： CHA was extracted, and the expression levels of Frizzled 3 and Frizzled 4 were analyzed by using real time PCR. The results showed that the ratio of expression level of Frizzled 3, Frizzled 4 between Cricetulusgriseus and A ： CHA were revealed to be increased by 4 and 5 times. These data demonstrated that the gene expression level of Frizzled 3, Frizzled 4 in the A：CHA were lower than that in the Cricetulusgri- seus. The resulting data suggests the notion the two genes may be contribute to theAlbino phenotype of A：CHA.

关 键 词：黑线仓鼠 白化突变系(A:CHA) FRIZZLED 3基因 FRIZZLED 4基因 实时荧光定量PCR 

分 类 号：S443.3[农业科学—农业昆虫与害虫防治] Q78[农业科学—植物保护]