葛根素下调TGFβ抑制乙醛诱导的肝星状细胞凋亡的机理研究  

Puerarin prevents hepatic stellate cells from acetaldehyde stimulated apoptosis through downregulating TGFβ

作　　者：张春燕[1] 陶忠桦 陈川宁[1] 甘淋[1] 

机构地区：[1]西南医科大学生物化学与分子生物学教研室,四川泸州646000 [2]西南医科大学公共卫生学院实验教学中心,四川泸州646000

出　　处：《时珍国医国药》2017年第4期790-793,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81341121);四川省卫生厅项目(No.120366);泸州市科技计划项目(泸市科函[2011]42号文件);泸州医学院自然科学项目(2012ZD-06;2011-55)

摘　　要：目的探讨葛根素对乙醛诱导的人肝星状细胞(HSC)的细胞增殖、细胞凋亡及TGFβ mRNA表达水平的影响。方法常规细胞培养人HSC,用乙醛诱导细胞凋亡;用乙醛、葛根素处理HSC,采用CCK-8方法检测细胞增殖,流式细胞术检测细胞凋亡,RT-PCR方法检测TGFβ mRNA表达水平。结果乙醛成功诱导HSC活化增殖,发生凋亡;葛根素(3.0mg/ml)明显抑制乙醛刺激的HSC增殖,抑制乙醛刺激HSC的细胞凋亡,显著降低细胞因子TGFβ的mRNA表达。结论葛根素抑制HSC活化增殖、通过降低TGFβ mRNA表达水平抑制乙醛诱导的凋亡和发挥抗酒精性肝硬化作用。Objective To explore the role of puerarin on cell proliferation, cell apoptosis and TGFβ mRNA expression in human hepatic stellate ceils（HSC） prevented with acetaldehyde. Methods Human HSCs were induced cell apoptosis by stimulating with acetaldehyde. HSCs prevented with acetaldehyde and puerarin, cell proliferation was detected by CCK -8 assay, cell apoptosis was analyzed by FACS, and TGFβ mRNA expression was assessed by RT - PCR. Results Acetaldehyde induced significantly ac- tivation, proliferation and apoptosis in HSC. Puerarin（ 3.0 mg/mL ） significantly inhibited cell proliferation, cell apoptosis and TGFβ mRNA expression stimulated by acetaldehyde in HSCs. Conclusion Puerarin repressed cell proliferation, apoptosis stimulated by acetaldehyde in human HSCs, and downregulated the mRNA expression of TGFβ , which played a role in preventing from hepatic fibrosis.

关 键 词：葛根素 乙醛 肝星状细胞 TGFΒ 凋亡 

分 类 号：R285.5[医药卫生—中药学]