Oasis MCX固相萃取柱结合内标法高效检测羊肉瘦肉精残留量的方法研究  

Study on the efficient determination of clenbuterol in lamb by using Oasis MCX SPE column combined internal standard method

作　　者：弓丽华 李万鹏 郭良起 曲良苗 师敬敬 单素敏 马伟伟[2] 张永辉[2] 

机构地区：[1]河南广电计量检测有限公司食品实验室,河南郑州450001 [2]郑州轻工业学院材料与化学工程学院,河南郑州450001

出　　处：《轻工学报》2017年第3期14-20,共7页Journal of Light Industry

基　　金：国家自然科学基金项目(21301158);国家大学生创新创业训练项目(201510462065)

摘　　要：在以乙腈-0.1%甲酸溶液为流动相、5%氨化甲醇为洗脱剂、梯度洗脱、HPLC-MS/MS电喷雾正离子、多反应监测模式的检测条件下,利用高效液相色谱串联三重四级杆质谱仪,研究了Oasis MCX固相萃取柱结合内标法检测羊肉中克伦特罗(俗称瘦肉精)残留量的方法.结果表明,克伦特罗残留量在线性范围0.050~5.000 ng/mL内均具有良好的检出率,相关系数为0.997 8,加标回收率为98%~102%,相对标准偏差为2.09%.该方法灵敏度高,检测结果准确度高,适用于羊肉中克伦特罗残留的定量检测,对其他肉质中克伦特罗的检测也具有一定的参考价值.The study aimed to establish the detection method of clenbuterol in lamb by using Oasis MCX SPEcolumn combined internal standard method. The separation was performed by using aacid solution as mobile phase and 5% ammoniated methanol as eluent. The detection was conducted by usingHPLC-ESI-MS/MS in positive ion and multiple reaction monitoring （ MRM） modes. The results showed thatthis method possessed satisfactory detecting rate for clenbuterol ranging from 0. 050 ng/mL to 5. 000 ng/mL.The coefficient was 0. 997 8, the recovery rate was 98% -102% ,and the relative standard deviation was2. 09%. With high sensitivity and accuracy, this method could be used for dand it also had certain guiding significance for quantitative analysis of clenbuterol in other meat products.

关 键 词：羊肉 瘦肉精 OASIS MCX固相萃取柱 内标法 

分 类 号：TS251[轻工技术与工程—农产品加工及贮藏工程]