新疆地区非小细胞肺癌中表皮生长因子受体基因突变与临床病理特征的关系  

Clinicopathologic characteristics of epidermal growth factor receptor mutations in non-small cell lung cancer in Xinjiang region

作　　者：师艺[1] 潘钲 崔文丽[1] 马志萍[1] 庞雪莲[1] 张巍[1] 马遇庆[1] 

机构地区：[1]新疆医科大学第一附属医院病理科,乌鲁木齐830011 [2]新疆维吾尔自治区职业病医院病理科,乌鲁木齐830091

出　　处：《中华病理学杂志》2017年第5期309-313,共5页Chinese Journal of Pathology

基　　金：基金项目：国家自然科学基金（81560035）;新疆维吾尔自治区自然科学基金面上项目（2014211C032）;新疆维吾尔自治区青年科技创新人才培养工程（qn2015bs011）;新疆维吾尔自治区博士后基金

摘　　要：目的 探讨新疆地区表皮生长因子受体（EGFR）基因点突变在非小细胞肺癌中与临床病理特征之间的关系.方法 收集2013年1月至2015年12月新疆医科大学第一附属医院病理科检测的582例非小细胞肺癌患者石蜡包埋组织,用Qiagen试剂盒提取DNA,经TaqMan探针荧光定量qRT-PCR技术检测非小细胞肺癌中EGFR基因第18、19、20、21号外显子共32个点突变,分析其突变情况与临床病理特征的相关性.结果 582例非小细胞肺癌患者的石蜡包埋组织中,EGFR基因点突变173例,突变率为29.7%（173/582）.患者女性（50.5%、98/194）、无吸烟史（47.3%、96/203）、高分化（6/9）、腺鳞癌（6/11）、周围型（34.9%、88/252）、手术标本（38.2%、83/217）EGFR基因突变率高,差异有统计学意义（P〈0.05）.Logistic回归多因素分析显示仅有变量性别、分化程度、病理类型对EGFR基因突变有统计学意义（P〈0.05）,其他变量与EGFR基因突变没有统计学意义.结论 非小细胞肺癌患者EGFR基因突变以女性、不吸烟、高分化腺癌中突变率较高,差异有统计学意义,其中性别、分化程度及病理类型为EGFR基因突变状况的独立影响因素.Objective To investigate the point mutation of epidermal growth factor receptor （EGFR） gene and clinicopathologic characteristics in patients with non-small cell lung cancers（NSCLC）of Xinjiang region. Methods Five-hundred and eighty-two cases of paraffin-embedded tissue in patients with NSCLC were collected between January 2013 and December 2015 in the First Affiliated Hospital of Xinjiang Medical University. The DNA was extracted from these tissues by Qiagen kit, to test thirty-two mutations in EGFR exons 18, 19, 20 and 21 using fluorescent quantitative qRT-PCR technology by TaqMan probe;the clinicopathologic features of patients were analyzed according to the mutation status of EGFR. Results There were 173 cases with EGFR gene mutation in 582 cases of paraffin-embedded tissue in patients with NSCLC, and the mutation rate was 29.7%（173/582）.There were statistical difference in female patients （50.5%,98/194）, no history of smoking（47.3%,96/203）, high differentiation（6/9）,adenosquamous carcinoma（6/11）, peripheral location （34.9%,88/252）, and surgical specimens（38.2%,83/217）, respectively （P〈0.05）. Multiple factors Logistic analysis showed that gender, degree of differentiation, and pathologic types had statistical differences to EGFR when α=0.05. There were no statistical differences between other variants.Conclusions There are higher rate EGFR gene mutation in women patients, non-smokers, and well-differentiated, adenocarcinoma. Gender, degree of differentiation and pathological patterns are independent influencing factors on EGFR mutation status.

关 键 词：癌 非小细胞肺 受体 表皮生长因子 DNA突变分析 聚合酶链反应 DNA探针 

分 类 号：R734.2[医药卫生—肿瘤]