Recent 5-year Findings and TechnologicalAdvances in the Proteomic Study of ,,HIV-associated Disorders  

作　　者：Lijun Zhang Xiaofang Jia Jun-O Jin Hongzhou Lu Zhimi Tan 

机构地区：[1]Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China

出　　处：《Genomics, Proteomics & Bioinformatics》2017年第2期110-120,共11页基因组蛋白质组与生物信息学报（英文版）

基　　金：funded by grants from the National Natural Science Foundation of China (Grant No. 81271834);the National High-tech R&D Program (863 Program; Grant No. 2014AA021403) of China;the Shanghai Technical Service Platform for the Detection of Pathogenic Microorganism (Grant No. 15DZ2290200), Shanghai, China

摘　　要：Human immunodeficiency virus-1(HIV-1) mainly relies on host factors to complete its life cycle. Hence, it is very important to identify HIV-regulated host proteins. Proteomics is an excellent technique for this purpose because of its high throughput and sensitivity. In this review, we summarized current technological advances in proteomics, including general isobaric tags for relative and absolute quantitation(i TRAQ) and stable isotope labeling by amino acids in cell culture(SILAC), as well as subcellular proteomics and investigation of posttranslational modifications.Furthermore, we reviewed the applications of proteomics in the discovery of HIV-related diseases and HIV infection mechanisms. Proteins identified by proteomic studies might offer new avenues for the diagnosis and treatment of HIV infection and the related diseases.Human immunodeficiency virus-1(HIV-1) mainly relies on host factors to complete its life cycle. Hence, it is very important to identify HIV-regulated host proteins. Proteomics is an excellent technique for this purpose because of its high throughput and sensitivity. In this review, we summarized current technological advances in proteomics, including general isobaric tags for relative and absolute quantitation(i TRAQ) and stable isotope labeling by amino acids in cell culture(SILAC), as well as subcellular proteomics and investigation of posttranslational modifications.Furthermore, we reviewed the applications of proteomics in the discovery of HIV-related diseases and HIV infection mechanisms. Proteins identified by proteomic studies might offer new avenues for the diagnosis and treatment of HIV infection and the related diseases.

关 键 词：HIV-1 PROTEOMICS Interaction i TRAQ SILAC 

分 类 号：R512.91[医药卫生—内科学]