云南高校图书馆联盟文献共享服务平台- SILAC

SILAC: 作品数：27被引量：46H指数：4; 导出分析报告; 相关领域：医药卫生生物学更多>>; 相关作者：徐平孙雪松易淑红陈智鹏翟贵金更多>>; 相关机构：军事医学科学院暨南大学武汉大学北京蛋白质组研究中心更多>>; 相关期刊：《生物化学与生物物理进展》《生物技术通讯》《Genomics, Proteomics & Bioinformatics》《中国药理学通报》更多>>; 相关基金：国家自然科学基金国家重点基础研究发展计划国家高技术研究发展计划中国科学院仪器设备功能开发技术创新项目更多>>

基于Super-SILAC定量技术系统解析槲皮素调控巨噬细胞的组蛋白翻译后修饰图谱: 《中国生物化学与分子生物学报》2024年第11期1574-1584,共11页刘志晴翟琳辉吉敬谭敏佳刘萍; 上海市优秀学术/技术带头人(No.22XD1420900)资助。; 槲皮素(quercetin,Que)是一种具有多种生物活性的黄酮类化合物,在自然界中分布广泛,具有抗炎和抗肿瘤等生物活性。研究发现,槲皮素的抗炎活性与其对组蛋白翻译后修饰的调控密切相关。然而,槲皮素调控组蛋白翻译后修饰发挥抗炎作用的精...; 关键词：槲皮素表观遗传组蛋白翻译后修饰细胞稳定同位素标记巨噬细胞

The synthesis of PROTAC molecule and new target KAT6A identification of CDK9 inhibitor i CDK9被引量：1: 《Chinese Chemical Letters》2023年第4期216-221,共6页Mingtao Ao Jun Wu Yin Cao Yaohui He Yuxiang Zhang Xiang Gao Yuhua Xue Meijuan Fang Zhen Wu; supported by grants from the National Key R&D Program of China (Nos. 2018YFA0107303 and 2020YFA0908100);the National Natural Science Foundation of China (Nos. 81773600and 82102746);the China Postdoctoral Science Foundation (No.2021M690095);the Fundamental Research Funds for the Central Universities (No. 20720180051)。; Cyclin-dependent kinases(CDKs) have become potential targets for treating various diseases, especially cancer. Compound i CDK9 is an excellent and selective CDK9 inhibitor, but its major limitation is the potential to...; 关键词：PROTAC SILAC iCDK9 KAT6A Target identification

A proteomic approach to better understand the role of human neutrophil peptides in the NSCLC microenvironment: 《Journal of Cancer Metastasis and Treatment》2023年第1期516-529,共14页Eline Berghmans Daniel Flender Evelien Smits Sofie Struyf Geert Baggerman; Aim:Although treatment of lung cancer,one of the deadliest diseases worldwide,with immune checkpoint inhibitors(ICIs)has shown promising outcomes,these survival outcomes are only observed in a relatively small subset ...; 关键词：Human neutrophil peptide 1(HNP1) in vitro cocultures PROTEOMICS secretome analysis SILAC

Identification of sitagliptin binding proteins by affinity purification mass spectrometry被引量：1: 《Acta Biochimica et Biophysica Sinica》2022年第10期1453-1463,共11页Xue-Ning Wang Byu-Ri Sim Hong Chen Yun-Xiao Zheng Jun-Biao Xue Lei Wang Wei-Sha Kong Kuan Zhou Shu-Juan Guo Jing-Li Hou Jiong Zhang He-Wei Jiang Sheng-Ce Tao; supported by the grants from the National Key Research and Development Program of China(No.2016YFA0500600);the National Natural Science Foundation of China(Nos.31900112,21907065,31970130,and 31670831);Anhui Provincial Natural Science Foundation(No.2108085QH380);Grants for Scientific Research from Anhui Medical University(Nos.0601094201 and 2021xkj012).; Type 2 diabetes mellitus(T2DM)is recognized as a serious public health concern with increasing incidence.The dipeptidyl peptidase-4(DPP-4)inhibitor sitagliptin has been used for the treatment of T2DM worldwide.Althoug...; 关键词：AP-MS drug-target interaction SITAGLIPTIN SILAC SPR type 2 diabetes mellitus

人上皮性卵巢癌细胞系侧群细胞的肿瘤干细胞样细胞生物学特性及膜蛋白差异表达的鉴定被引量：5: 《基础医学与临床》2022年第2期221-227,共7页尹婕潘凌亚温仪萍黄虹曾靖李晓莹韩甜甜金滢李艳; 国家自然科学基金(30772315,81572564)。; 目的鉴定上皮性卵巢癌细胞侧群细胞(SP细胞)肿瘤干细胞样细胞生物学特性,并检测侧群细胞内差异蛋白质的表达。方法流式分选上皮性卵巢癌细胞系SKOV3和A2780具有外泌Hochest33342染料生物学特性的侧群细胞,鉴定其肿瘤干细胞样细胞相关生...; 关键词：卵巢癌肿瘤干细胞样细胞侧群细胞表面标记蛋白细胞培养稳定同位素标记(SILAC)

Comprehensive profiling of CTP-binding proteins using a biotinylated CTP affinity probe: 《Chinese Chemical Letters》2021年第11期3479-3482,共4页Mengting Pa Yunming Liu Xiaofang Zheng Meijuan Zhou Changjun You Xiaoxia Dai; supported by the National Natural Science Foundation of China(Nos.21807030,21907028);the Science and Technology Innovation Program of Hunan Province(No.2019RS2020);Natural Science Foundation of Hunan Province(No.2020JJ5046);the Fundamental Research Funds for the Central Universities(Nos.531118010061,531118010259).; Recent studies have shown that CTP may act as a ligand to regulate the activity of its target proteins in many biological processes.However,proteome-wide identification of CTP-binding proteins remains challenging.Here...; 关键词：CTP-binding proteins CTP affinity probe Mass spectrometry SILAC Quantitative proteomics

Nsp2 has the potential to be a drug target revealed by global identification of SARS-CoV-2 Nsp2-interacting proteins被引量：3: 《Acta Biochimica et Biophysica Sinica》2021年第9期1134-1141,共8页Yun-Xiao Zheng Lei Wang Wei-Sha Kong Hong Chen Xue-Ning Wang Qingfeng Meng Hai-Nan Zhang Shu-Juan Guo He-Wei Jiang Sheng-Ce Tao; the grants from the National Key Research and Development Program of China(No.2016YFA0500600);the National Natural Science Foundation of China(Nos.31900112,21907065,31970130,and 31670831).; Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has become a global health threat since December 2019,and there is still no highly effective drug to control the pandemic.To facilitate drug target identifica...; 关键词：NSP2 SARS-CoV-2 protein-protein interaction AP-MS SILAC

利用ChIP-seq/SILAC技术筛选KLF15的靶向基因SUMO1: 《中华肾病研究电子杂志》2018年第2期74-81,共8页李鸥徐华马倩王旭尹忠吴玲玲谢院生蔡广研陈香美洪权; 国家自然科学基金(81470949,81330019); 目的利用ChIP-seq和SILAC-LC/MS技术寻找Krupple样因子(KLF15)的靶向基因及其结合位点。方法 (1)利用染色质免疫沉淀技术(ChIP)特异性富集并纯化人原代肾系膜细胞(HRMC)中可与KLF15结合的DNA片段,通过高通量测序技术(Hi Seq)检测和分析...; 关键词：Krupple样因子15 肾系膜细胞染色质免疫沉淀联合高通量测序技术稳定同位素标记细胞培养技术小类泛素样修饰分子1

Recent 5-year Findings and TechnologicalAdvances in the Proteomic Study of ,,HIV-associated Disorders被引量：1: 《Genomics, Proteomics & Bioinformatics》2017年第2期110-120,共11页Lijun Zhang Xiaofang Jia Jun-O Jin Hongzhou Lu Zhimi Tan; funded by grants from the National Natural Science Foundation of China (Grant No. 81271834);the National High-tech R&D Program (863 Program; Grant No. 2014AA021403) of China;the Shanghai Technical Service Platform for the Detection of Pathogenic Microorganism (Grant No. 15DZ2290200), Shanghai, China; Human immunodeficiency virus-1(HIV-1) mainly relies on host factors to complete its life cycle. Hence, it is very important to identify HIV-regulated host proteins. Proteomics is an excellent technique for this purpos...; 关键词：HIV-1 PROTEOMICS Interaction i TRAQ SILAC

基于定量蛋白质组学分析迭鞘石斛对肺癌细胞的抑制作用及机理被引量：1: 《应用与环境生物学报》2017年第1期100-104,共5页张华李莹刘静何涛蒋伟淳泽胡亚东; 四川省"十三五"中药材育种攻关项目(2016NYZ0036-1);四川省应用基础研究项目(2014JY0179;2015JY0265);四川省科技支撑计划项目(2014FZ0086;2016JZ0015);四川省科技基础条件平台项目资助~~; 以基于SILAC(Stable isotope labeling with amino acids)的定量蛋白质组学为主要研究方法,通过精确定量蛋白质含量并进行差异分析,从蛋白层面系统研究迭鞘石斛(Dendrobium denneanum)乙醇提取物对人肺癌细胞A549的影响.结果发现迭鞘石...; 关键词：迭鞘石斛肺癌细胞作用机理 SILAC 定量蛋白质组

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