玉米黄色素提取工艺优化及其抗癌活性研究  

Extraction process optimization and anticancer activity of maize yellow pigment

作　　者：于慧[1] 刘美佳[1] 刘剑利[1] 曹向宇[1] 

机构地区：[1]辽宁大学生命科学院,辽宁沈阳110036

出　　处：《食品与机械》2017年第3期139-144,共6页Food and Machinery

基　　金：国家自然科学基金委员会科学部主任基金项目(编号:31240005);辽宁省农业领域青年科技创新人才培养资助计划项目(编号:2015013);辽宁省高等学校优秀人才支持计划项目(编号:LJQ2013002);辽宁省高等学校科学研究一般项目(编号:L2014007);辽宁大学博士科研启动项目

摘　　要：探究超声辅助酶法制备玉米黄色素的最佳工艺,以及玉米黄色素对人胃腺癌细胞(BGC-823)生长的抑制作用。通过单因素和响应面试验,探讨超声辅助酶法制备玉米黄色素的最佳条件;用制备的玉米黄色素处理BGC-823细胞,并进行形态学观察和MTT法细胞存活率检测,初步考察其抗癌活性。超声辅助酶法提取玉米黄色素的最佳条件为:酶活力10 000U/g,超声功率400 W,酶解温度35℃,该条件下提取率达最大,为(72.64±0.42)μg/g;细胞形态学观察及MTT细胞活力检测显示玉米黄色素对BGC-823细胞具有一定的生长抑制作用。超声辅助酶法提取玉米黄色素是一种有效的提取方法,制备的玉米黄色素对BGC-823细胞生长有较强的抑制作用。Objective：To study the best preparation technology of maize yellow pigment by ultrasonic assisted with enzymatic method,and the inhibitory effect of them on the growth of human gastric cancer cells（BGC-823）.Methods：The optimum conditions of maize yellow pigment were extracted by ultrasonic assisted with enzymatic method by single factor and response surface experiments.The inhibition of maize yellow pigment on BGC-823 cells was detected by morphological observation and MTT assay to evaluate the antitumor activity.Results：the maize yellow pigment best extraction condition of ultrasonic with enzymatic method is：enzyme activity 10 000U/g,ultrasonic power 400 W,enzymolysis temperature 35 ℃,the maximum extraction yield was（72.64±0.42）μg/g under the condition,the maize yellow pigment has a certain inhibitory effect on BGC-823 cell were studied by cell morphological observation and MTT test.Conclusion：The extraction of maize yellow pigment by ultrasonic assisted with enzymatic method is a kind of effective extraction method,the maize yellow pigment prepared has strong inhibitory effect on BGC-823 cells.

关 键 词：玉米黄色素 超声波 酶法 抗癌 

分 类 号：TS213.4[轻工技术与工程—粮食、油脂及植物蛋白工程]