EGCG阻断FZD3和MAPK10基因表达调控颌骨牙源性角化囊性瘤转归  

The role of blocking of FZD3 and MAPK10 with EGCG in the treatment of jaw keratocystic odontogenic tumor

作　　者：杨银辉 王耀钟 袁荣涛[2] 贾暮云[2] 林锡江 

机构地区：[1]青岛市口腔医院口腔颌面外科,山东青岛266003 [2]青岛大学附属医院口腔颌面外科,山东青岛266003

出　　处：《口腔医学》2017年第5期403-407,共5页Stomatology

基　　金：青岛市公共领域科技支撑计划项目[11-2-3-1-(20)-nsh];山东省自然科学基金面上项目(ZR2016HM34)

摘　　要：目的研究Wnt信号通路阻断剂表没食子儿茶素没食子酸酯(epigallocatechin-3-gallate,EGCG)在颌骨牙源性角化囊性瘤开窗灌洗治疗中的调控作用。方法收集颌骨牙源性角化囊性瘤标本25例,其中开窗灌洗治疗前病例20例,开窗灌洗治疗术后病例5例,3例生理盐水灌洗,2例EGCG灌洗。应用实时荧光定量PCR及免疫组化方法,检测Wnt信号通路相关基因FZD3及MAPK10的表达,以正常口腔黏膜作为对照。结果 FZD3和MAPK10基因在颌骨牙源性角化囊性瘤中的表达高于口腔正常黏膜,应用生理盐水开窗灌洗治疗后,FZD3和MAPK10的表达降低,但仍高于正常口腔黏膜,而应用EGCG开窗灌洗治疗后,FZD3和MAPK10的表达与正常黏膜差异不大。结论 FZD3和MAPK10在颌骨牙源性角化囊性瘤中高表达,应用EGCG开窗灌洗治疗可使FZD3和MAPK10的表达降低并趋于正常。Objective To study the role of epigallocatechin-3-gallate（ EGCG）, the inhibitor of Wnt signaling pathway, in the marsu- pialization treatment of jaw keratocystic odontogenic tumor（KCOT）. Methods Twenty-five specimens of KCOT were obtained, twenty before marsupialization, five after marsupialization, three treated with EGCG solution and two with normal saline（NS）. The expressions of FZD3 and MAPKI0, Wnt signaling pathway genes, were detected. The normal oral mucosa of the same patient was as control. Re- sult Compared to normal oral mucosa, the expressions of FZD3 and MAPK10 in KCOT were up-regulated, which decreased after the treatment of marsupialization with normal saline, but were still higher than those of normal oral mucosa. However, the expressions of FZD3 and MAPKIO in KCOT sharply decreased after the treatment with EGCG and were similar to normal oral mucosa. Conclusion FZD3 and MAPKI0 genes are over-expressed in KCOT. Marsupialization with EGCG can block the expressions of FZD3 and MAPK10, which play a role in the treatment of jaw KCOT.

关 键 词：牙源性角化囊性瘤 表没食子儿茶素没食子酸酯 WNT信号通路 

分 类 号：R739.82[医药卫生—肿瘤]