RASD1在胶质瘤U251细胞迁移中的作用  

The role of RASD1 in the migration of glioma U251 cells

作　　者：孙宗汉[1] 高尚锋[2,3] 刘光平[1] 王杰[1] 华磊[2] 朱玉辐[2] 于如同[2,3] 

机构地区：[1]徐州医学院研究生学院,徐州221004 [2]徐州医学院附属医院脑科医院 [3]徐州医学院神经系统疾病研究所

出　　处：《临床神经外科杂志》2017年第2期98-101,共4页Journal of Clinical Neurosurgery

基　　金：国家自然科学基金(31400930;81302175);江苏省自然科学基金(BK20140217);中国博士后科学基金(2015M570480);江苏省博士后科研资助计划(1302111B)

摘　　要：目的揭示Ras家族小G蛋白——RASD1在胶质瘤U251细胞迁移中的作用。方法在稳定过表达RASD1的胶质瘤U251细胞系中,应用Western blot验证RASD1过表达效果;应用鬼笔环肽染色显示细胞骨架蛋白F-actin的分布;应用细胞划痕实验检测过表达RASD1对胶质瘤U251细胞迁移的影响。结果胶质瘤U251细胞系中外源性RASD1过表达成功。过表达RASD1降低了细胞骨架蛋白F-actin在细胞板状伪足、侵袭伪足上分布。稳定过表达RASD1的胶质瘤U251细胞迁移能力下降。结论 RASD1具有抑制胶质瘤U251细胞迁移能力的作用。Objective To reveal the role of dexamethasone-induced Ras-related protein 1（RASD1）,one of the Ras family small G proteins,in the migration of glioma U251 cell. Methods Western blot was performed on the U251 cell line to verify the overexpression of RASD1;PhalloidinF-actin immunofluorescence was performed to observe the distribution of cytoskeletal protein F-actin;and cell wound scratch assay was performed to evaluate the effect of RASD1-overexpression on the migration of U251 cells. Results Exogenous RASD1 was successfully overexpressed in U251 cells,which reduced the accumulation of F-actin in the lamellipodiaand invasion-filopodia,and inhibited the migratory ability of U251 cells. Conclusion RASD1 may inhibit the migration of glioma U251 cells.

关 键 词：RASD1 胶质瘤 F-ACTIN 迁移 

分 类 号：R739.4[医药卫生—肿瘤]