甘薯IbANR基因的多态性分析  

Polymorphic analyses of IbANR gene from sweetpotato

作　　者：武传建[1] 戚骁 李仁赛 封功成 苏静静[1] 葛海军[1] 马代夫[1,2] 王爱民[1,2] 

机构地区：[1]江苏师范大学生命科学学院,江苏徐州221116 [2]江苏徐淮地区徐州农业科学研究所,江苏徐州221131

出　　处：《江苏师范大学学报（自然科学版）》2017年第1期30-33,45,共5页Journal of Jiangsu Normal University：Natural Science Edition

基　　金：江苏省普通高校研究生科研创新计划项目(KYZZ15-0388);江苏师范大学生命科学学院2016年大学生实践创新训练计划项目

摘　　要：花青素还原酶(ANR)是原花青素生物合成的关键酶,对甘薯色素的积累起重要作用.为了探讨甘薯花青素还原酶基因(IbANR)的多态性,根据徐紫薯3号cDNA序列设计特异引物,从27个不同的甘薯品种(品系)中扩增出IbANR基因序列,在编码区中发现72个变异位点,其中39个为SNP位点,检出频率为1SNP/488bp;内含子区变异出现的频率约为编码区的3倍.推测氨基酸序列也存在多态性.本研究为甘薯IbANR基因的功能研究及其在甘薯分子辅助育种中的应用提供支持.Anthocyanidin reductase is a key enzyme in proanthocyanidins biosynthesis pathway.To explore the polymorphism of IbANRgene in sweetpotato,primers designed according to cDNA sequence of Xuzishu No.3 were uesd to amplify part DNA sequences of IbANRgene from 27 varieties of sweetpotato.The results of alignment and sequence analysis showed that total 72 mutate sites were found in the coding region.And among 72 mutations there were 39 SNPs with the frequency of 1 SNP/488 bp.The SNPs frequency in introns was about 3 times of that in coding region.Polymorphisms of putative amino acid were also found.These data might give support to function research of IbANRand application in molecular assistance breeding of sweetpotato.

关 键 词：甘薯 IbANR SNP位点 INDEL 

分 类 号：Q751[生物学—分子生物学] S531[农业科学—作物学]