青海汉族和藏族高龋患者口腔微生物多样性差异研究  

Study on the oral microbial diversity differences between Han and Tibetan people with high caries risk in Qinghai

作　　者：李志艳[1] 赵翀[1] 刘静[2] 张欣[2] 朱德锐[2] 陈筠[1] 

机构地区：[1]青海大学附属医院口腔内科,青海西宁810001 [2]青海大学医学院基础医学研究中心,青海西宁810016

出　　处：《牙体牙髓牙周病学杂志》2017年第5期250-253,265,共5页Chinese Journal of Conservative Dentistry

基　　金：青海省科技计划项目(2014ZJ759);青海大学附属医院中青年科技计划项目(ASRF201406)

摘　　要：目的:采用高通量测序研究青海汉、藏族高龋患者口腔疾病微生物的多样性和群落差异。方法:选择我院口腔内科门诊汉、藏高龋患者各10例唾液样本,利用Illumina MiSeq平台进行细菌16S rRNA基因(V3-V4)区高通量测序,并利用Mothur软件分析微生物的群落结构和多样性。结果:基于97%的相似性类聚获得汉、藏高龋患者口腔微生物物种注释(Operational taxonomic unit,OTU)数目分别为51和30。汉族组分类学地位明确的有4门7纲28属,藏族组有2门4纲22属。汉族高龋组有12种优势种群,依次是:假单胞菌属(32.52%)、肠杆菌目未分类菌属(29.91%)、明串珠菌属(10.56%)、肠膜菌属(4.71%)、乳球菌属(3.71%)和链球菌属(3.04%)等。藏族组的优势菌主要有4个,依次是TM7-3未知菌属(55.86%)、未知菌属SR1(20.36%)、黄色单胞菌目未分类菌属(17.38%)和假绿黄单胞菌(2.46%)。结论:青海汉族龋患者群的唾液微生物多样性与丰度明显高于藏族组,且优势微生物种群亦存在明显差异。AIM To study the difference of the diversity and composition of oral microorganism between Han and Tibetan people with high caries risk in Qinghai. METHOD： 10 Han Chinese and 10 Tibetans with high caries risk were selected from outpatients. High - throughput sequencing method was performed by using an Illumina miseq sequencing platform for studying bacterial 16S rRNA gene V3 - V4 region, then the microbial community structure and diversity were analyzed with the Mothur software. RESULTS ： Based on 97 % similarity clustering, the numbers of Op-erational Taxonomic Units （OTUs） in Han and Tibetan groups were 51 and 30 respectively. Oral microorganisms in the Han group were classified into 4 phyla, 7 classes and 36 genera, and those in the Tibetan group were classified into 2 phyla, 4 classes and 27 genera. The 12 dominant species in the Han group included Pseudomonas （ 32. 52% ）, Enterobacteriaceae unclassified （ 29. 91%）,Leuconostoc （10. 56%）,Mesenteroides （ 4. 71%）,Lactococcus （3.71% ） and Streptococcus （ 3. 04% ） , et al,4 dominant species in the Tibetan group were Bacteria TM7 - 3 （55. 86% ） , Bacteria SRI （20. 36% ） , Xanthomonadaceae unclassified （ 17. 38% ） and Pseudomonas （2. 46% ）. CONCLUSION： The oral microbial diversity and abundance in the Han group are significantly higher than those in the Tibetan group, there is a significant difference in the dominant oral microorganisms between the Han and Tibetan group in Qinghai region.

关 键 词：青海 藏族 龋病 高通量测序 微生物多样性 

分 类 号：R780.2[医药卫生—口腔医学]