田菁来源半乳甘露低聚糖对青春双歧杆菌的增殖  

In vitro proliferation of Bifidobacterium adolescentis by galactomanno-oligosaccharides from Sesbania cannabina

作　　者：陆旻妍 杨磊[1,2] 赖晨欢[1,2] 周梦怡[1] 奚晓桐 余世袁[1,2] 勇强[1,2] 

机构地区：[1]南京林业大学林业资源高效加工利用协同创新中心 [2]南京林业大学化学工程学院,南京210037

出　　处：《林业工程学报》2017年第3期64-69,共6页Journal of Forestry Engineering

基　　金：国家林业公益性行业科研专项(201404615);江苏省前瞻性联合研究项目(BY2015006-05);江苏省重点研发计划(BF2015007);江苏高校自然科学研究项目(15KJB530009)

摘　　要：田菁是被广泛应用的盐碱地改良先锋植物,半乳甘露聚糖是田菁种子的主要成分。半乳糖、甘露糖和半乳甘露低聚糖为半乳甘露聚糖酶法完全和不完全水解产物。为揭示不同分子质量半乳甘露聚糖不完全降解产物的生物学功能差异,笔者研究了半乳糖、甘露糖和不同分子质量分布的半乳甘露低聚糖对青春双歧杆菌(Bifidobacterium adolescentis)的增殖作用及其代谢产物。青春双歧杆菌分别以3g/L(质量浓度)半乳糖和甘露糖为碳源培养24h,糖利用率分别为99.67%和72.00%,菌体质量浓度从初始的0.04g/L分别增殖到1.82和0.39g/L,生成的总短链脂肪酸(乙酸、乳酸和丙酸)质量浓度分别为6.76和3.02g/L。不同分子质量分布的半乳甘露低聚糖能有效增殖青春双歧杆菌,半乳甘露低聚糖平均分子质量越低,对青春双歧杆菌的增殖作用越强。青春双歧杆菌以3g/L,分子质量分布为510,25和≤2ku的半乳甘露低聚糖为碳源培养24h,半乳甘露低聚糖利用率分别为59.00%,61.67%和86.33%,青春双歧杆菌菌体质量浓度从初始浓度的0.04g/L分别增殖到0.51,0.79和1.71g/L。青春双歧杆菌以分子质量≤2ku的半乳甘露低聚糖为碳源培养24h,代谢产物乙酸、乳酸和丙酸的质量浓度分别为4.14,2.48和0.81g/L。Sesbania cannabina is widely used as a pioneer plant in improving saline alkali land, and galactomannan is the main component of S. cannabina seeds. Galactose, mannose and galactomanno-oligosaccharides are complete and incomplete degradation products of galactomannan. In order to reveal the differences in the biological functions of incomplete degradation products of galactomannan with different molecular weight, galactose, mannose and galactoman- no-oligosaccharides with different molecular weight distribution were used as carbon source for in vitro proliferation of Bifidobacteria adolescentis, and the main metabolites were analyzed. When 3 g/L of galactose and mannose was used as carbon source for proliferation of B. adolescentis, the sugar consumption were 99. 67% and 72. 00%, respectively, the cell concentration increased from 0. 04 g/L （ initial concentration） to 1.82 and 0. 39 g/L, and the production of total short chain acids（ acetic acid, lactic acid and propionic acid） were 6. 76 and 3.02 g/L, respectively. The galactomanno-oligosaccharides with different molecular weight distribution could proliferate B. adolescentis effectively, and the lower molecular weight of the galactomanno-oligosaccharides, the greater effect on the growth of the B. adolescen- tis. When 3 g/L galactomanno-oligosaccharides with the molecular weight distribution of 5-10 ku, 2-5 ku and the molecular weight less than 2 ku were used as carbon source for proliferation of B. adolescentis for 24 h, respectively, the consumption of galactomanno-oligosaccharides were 59. 00%, 61.67% and 86. 33%, the B. adolescentis concentration increased from initial concentration of 0. 04 g/L to 0. 51, 0. 79 and 1.71 g/L, respectively. When 3 g/L galactomanno-oligosaccharides with the molecular weight less than 2 ku was used as carbon source for proliferation of B. adolescentis for 24 h, the metabolites concentration of acetic acid, lactic acid and propionic acid were 4.41, 2.48 and 0. 81 g/L, respectively.

关 键 词：半乳甘露低聚糖 田菁 青春双歧杆菌 增殖 短链脂肪酸 

分 类 号：Q53[生物学—生物化学] TQ921[轻工技术与工程—发酵工程]