糖的吡唑啉酮柱前衍生化方法及其在海洋糖分析中的应用  

The application of carbohydrates pre-column pyrazolone labeling on marine carbohydrates analysis

作　　者：蒋宏飞[1] 于日磊[1] 万升标[1] 江涛[1] 

机构地区：[1]中国海洋大学海洋药物教育部重点实验室医药学院,山东青岛266003

出　　处：《中国海洋药物》2017年第2期91-96,共6页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目(81373322)资助

摘　　要：糖类物质作为细胞组成中最为重要的物质之一,是蛋白质、脂肪代谢的必要物质,也是人体必需的营养元素之一。但人们对糖类化物质在生物体内的功能作用的研究却很滞后,这主要是因为糖类物质种类繁多、结构较为复杂且具有多种异构体,存在微观的不均一性。为此,常采用吡唑啉酮类化合物衍生化的方法处理,将糖类物质组分转变成为带有发色基团的分子,然后再进行紫外检测。糖类物质在海洋生物中广泛存在,吡唑啉酮衍生物衍生化方法是对海洋生物中糖类物质进行分析的重要手段。Carbohydrates are one of the most important substances of the cell,they are necessary intimates in the metabolic processes of protein and fat and they are also one of the substances that are necessary to humans.But humans＇ studies of carbohydrates are far form enough.The reasons are as follows：there are various kinds of carbohydrates,their structures are complicated,many kinds of isomers usually coexist and the microcosmic heterogeneity.So derivation of carbohydrates with pyrazolone derivatives to attach them with fluorescent are usually performed before carbohydrates analysis using UV detections.Carbohydrates widely exist in marine organisms,and derivation with pyrazolone derivatives is an important method for the analysis of carbohydrates of marine organisms.

关 键 词：糖类物质 吡唑啉酮 海洋生物 

分 类 号：R914[医药卫生—药物化学]