套细胞淋巴瘤中IgH/CCND1融合基因及细胞周期相关蛋白的表达  

Expression of IgH/CCND1 fusion gene and cell cycle associated protein in mantle cell lymphoma

作　　者：陈顺平[1] 吴文乔[1] 沈洪武[1] 邹宗楷[1] 苏海燕[1] 纪思灵[1] 洪少君 

机构地区：[1]福建医科大学附属漳州市医院病理科,363000

出　　处：《临床与实验病理学杂志》2017年第5期511-514,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：福建省卫生计生委青年科研课题(2014-2-45)

摘　　要：目的探讨细胞周期相关蛋白Cyclin D1、CDK4、p16蛋白及Ig H/CCND1融合基因在套细胞淋巴瘤(mantle cell lymphoma,MCL)中的表达及相互关系。方法采用荧光原位杂交(fluorescence in situ hybridization,FISH)法及免疫组化En Vision两步法联合检测40例已利用基因重排技术及免疫组化确诊的MCL石蜡包埋组织(实验组)及20例淋巴结反应性增生的石蜡包埋组织(对照组),并利用对照组建立MCL的Ig H/CCND1融合基因阈值。结果实验组中Cyclin D1蛋白阳性率为100%,CDK4蛋白阳性率为87.50%,p16蛋白阳性率为17.50%;实验组中Ig H/CCND1融合基因的阳性率为100%;对照组中Ig H/CCND1融合基因均阴性。结论 MCL细胞周期调控中Cyclin D1-CDK4-p16通路关系符合肿瘤细胞周期调控原理的阐述;采用FISH技术建立检测Ig H/CCND1融合基因阈值,为国内该融合基因的FISH法判断提供依据。Purpose To investigate the expression of cell cycle related protein including Cyclin D1, CDK4, p16 and IgH/ CCND1 fusion gene in mantle cell lymphoma （MCL） and their relationship with each other. Methods The expression of cell cycle related protein including Cyelin D1, CDK4, p16 and IgH/ CCND1 fusion gene were detected on the 40 cases of MCL （ expreimental group） and 20 cases of reactive hyperplasia （ control group） by using the combined detection of fluorescence in situ hybridization （FISH） and immunohistochemistry of EnVision two methods. 40 cases of MCL were confirmend by using gene rearrangement technique and immunohistochemistry. The threshold of IgH/CCND1 fusion gene of MCL was established in the control group. Results In the experimental group, Cyclin D1 protein positive expression rate was 100% , the positive expression of CDK4 protein rate was 87.50% , p16 protein positive expression rate was 17.50%. Positive rate of IgH/CCND1 fusion gene of 100%. These cell cycle related protein and IgH/CCND1 fusion gene were negative in the control group. Conclusion In MCL, Cyclin D1-CDK4-p16 pathway is consistent with the principle of tumor cell cycle regulation. The establishment of threshold value of IgH/CCND1 fusion gene by FISH technique may provide the basis for the judgement of FISH of the IgH/CCND1 in China.

关 键 词：套细胞淋巴瘤 CCND1 融合基因 荧光原位杂交 

分 类 号：R733[医药卫生—肿瘤]