γ-生育三烯酚抑制人胃腺癌SGC-7901细胞侵袭和迁移及其机制探讨  

Inhibitory effects of γ-tocotrienol on invasion and migration of human gastric cancer SGC-7901 cells and its mechanism

作　　者：张亚会[1] 沈双[2] 屠越华[1] 葛声[1] 王海霞[1] 田文霞[1] 孙文广[1] 

机构地区：[1]上海交通大学附属第六人民医院临床营养科,上海200233 [2]上海交通大学附属第六人民医院东院中心实验室,上海201306

出　　处：《肿瘤》2017年第5期441-447,共7页Tumor

基　　金：国家自然科学基金面上项目(编号:81273061)~~

摘　　要：目的:探讨γ-生育三烯酚(γ-tocotrienol,γ-T3)对人胃腺癌SGC-7901细胞侵袭和迁移的抑制作用及其可能的分子机制。方法:用不同浓度(0、15、30、45、60和100μmol/L)的γ-T3作用SGC-7901细胞后,采用CCK-8法、细胞划痕愈合实验和Transwell侵袭实验分别检测细胞增殖、迁移和侵袭能力的变化,然后采用蛋白质印迹法检测细胞中环氧合酶2(cyclooxygenase-2,COX-2)和核因子κB(nuclear factor-kappa B,NF-κB)信号通路蛋白的表达。结果:15~100μmol/Lγ-T3作用SGC-7901细胞24、48和72 h后,细胞增殖能力受到明显抑制,且呈时间和剂量依赖性(P值均<0.01)。15~60μmol/Lγ-T3单独作用或与10 ng/mL肿瘤坏死因子α(tumor necrosis factor-alpha,TNF-α)联合作用SGC-7901细胞24 h后,细胞迁移和侵袭能力均被明显抑制(P值均<0.01)。15~60μmol/Lγ-T3作用SGC-7901细胞24 h后,NF-κB、NF-κB p65和COX-2蛋白的表达水平均随着作用浓度的增加呈明显降低趋势(P值均<0.01)。结论:γ-T3可抑制人胃腺癌SGC-7901细胞的侵袭和转移,其作用机制可能与阻滞NF-κB信号通路和下调COX-2蛋白表达有关。Objective： To investigate the effects of ~,-tocotrienol （~,-T3） on the invasion and migration of human gastric cancer SGC-7901 cells as well as its possible molecular mechanism.Methods： The different concentrations （0, 15, 30, 45, 60, and 100μmol/L） of γ-T3 were used to treat SGC-7901 cells. Then the proliferation, migration and invasion of SGC-7901 cells were detected by CCK-8 assay, cell scratch wound healing assay and Transwell invasion assay, respectively. Furthermore, the expressions of cyclooxygenase-2 （COX-2） and nuclear factor-kappa B （NF-κB） signal pathway proteins were detected by Western blotting. Results： After treatment with different concentrations （1 5-100μmol/L ） of γ-T3 for 24, 48 and 72 h, the proliferation of SGC-7901cells was evidently inhibited in a time- and dose-dependent manner （all P 〈 0.01）. After treatment with γ-T3 （1 5-60 μmol/L） alone or combined with tumor necrosis factor-alpha （TNF-α） （10 ng/mL） for 24 h, the migration and invasion abilities of SGC-7901 cells were significantly inhibited （all P 〈 0.01）. The expressions of NF-κB, NF-κB p65 and COX-2 proteins were significantly down-regulated in SGC-7901 cells after γ-T3 （1 5-60μmol/L） treatment for 24 h （all P 〈 0.01）. Conclusion： γ-T3 can inhibit the invasion and migration of human gastric cancer SGC-7901 cells. Its mechanism may be associated with blocking NF-κB signal pathway and reducing the expression of COX-2 protein.

关 键 词：胃肿瘤 生育三烯酚类 肿瘤浸润 细胞迁移分析 NF-ΚB 

分 类 号：R735.2[医药卫生—肿瘤]