引药米酒对瓜蒌薤白体外抗氧化活性及体内药效学影响的研究  

Study the Effect of Rice Wine as Cites Drug on Oxidation Resistance in vitro and Pharmacodynamics in vivo of Gualou-Xiebai

作　　者：邹纯才[1] 肖敏[1] 鄢海燕[1] 孙婷婷[1] 徐启祥 宗倩妮 李楠[1] ZOU Chun-cai XIAO Min YAN Hai-yan SUN Ting-ting XU Qi-xiang ZONG Qian-ni LI Nan(Pharmacy School of Wannan Medical College,Wuhu 241002,China)

机构地区：[1]皖南医学院药学院,安徽芜湖241002

出　　处：《中药材》2016年第11期2605-2609,共5页Journal of Chinese Medicinal Materials

基　　金：安徽高校省级自然科学研究重大项目(KJ2015ZD41;KJ2016SD60);安徽省科技攻关项目(12010402089);安徽省大学生创新创业训练计划项目(201510368114)

摘　　要：目的:探讨米酒作为引药对瓜蒌薤白体外抗氧化活性及对ISO所致大鼠急性心肌缺血损伤药效学的影响。方法:制备瓜蒌薤白水提物的米酒溶液和水溶液,采用紫外-可见分光光度法测定瓜蒌薤白米酒溶液和水溶液的抗氧化活性。采用ISO制备大鼠急性心肌缺血模型。大鼠随机分为9组,每组8只,正常对照组、模型组分别给予生理盐水,瓜蒌薤白米酒溶液和水溶液按高(5 g/kg)、中(10 g/kg)、低(20 g/kg)剂量给药,复方丹参滴丸组剂量为0.085 g/kg,1次/d,给药7 d,末次给药后,除正常对照组外,各组造模并记录心电图变化,测定血浆及心肌组织NO、T-SOD、GSH-Px、MDA、CAT含量,HE染色观察心肌组织病理学变化。结果:瓜蒌薤白米酒溶液和水溶液的体外抗氧化活性差异无统计学意义。与模型组比较,各给药组大鼠心电图ST段显著降低(P<0.01或P<0.05),血浆及心肌组织NO、T-SOD、GSH-Px、MDA、CAT不同程度改善。与瓜蒌薤白水溶液组比较,瓜蒌薤白米酒溶液组对血浆及心肌组织NO、CAT指标的改善更显著。结论:引药米酒对瓜蒌薤白体外抗氧化活性无显著影响,但可促进瓜蒌薤白体内抗心肌缺血药效的发挥。Objective：To study the effect of rice wine as cites drug on oxidation resistance of Gualou-Xiebai in vitro and pharmacodynamics of ISO induced acute myocardial ischemia in rats.Methods：The rice wine solution and aqueous solution of Gualou-Xiebai water extract were prepared,and the oxidation resistances were determined by UV-Vis spectrophotometry.The model of acute myocardial ischemia in rats was made by ISO.Rats were randomly divided into nine groups,with eight rats in each group,the normal control group and the model groups were given normal saline respectively,Gualou-Xiebai rice wine solution group and aqueous solution were administered by high dose（5 g/kg）,middle dose（10 g/kg） and low dose（20 g/kg） correlation drug,the dosage of Compound Danshen Dripping pill group was 0.085 g/kg,the drugs were given once a day for seven successive days,after the last administration,in addition to the normal control group,the model was made and recorded by ECG change.The content of NO,T-SOD,GSH-Px,MDA,CAT and in plasma and myocardial tissue were measured,and the changes of myocardial tissue were observed by HE staining.Results：There was no significant difference in the antioxidant activity in vitro between rice wine solution group and aqueous solution group.Compared with model group,the ST segment of electrocardiogram of rats in each drug group were significantly decreased（P〈0.01 or P〈0.05）,and the indicators of NO,T-SOD,GSH-PX,MDA,CAT in the plasma and myocardial tissue were improved.Compared with aqueous solution group,the rice wine solution of Gualou-Xiebai improved NO,CAT indicators in plasma and myocardial tissue,and there was a significant difference of the content in increasing of the plasma levels of NO in plasma and CAT in myocardial tissue（P〈0.01 or P〈0.05）.Conclusion：Rice wine as cites drug does not has a significant effect on oxidation resistance of Gualou-Xiebai in vitro,but cites drug can promote the display the effect of anti-myocardial ischemia in vivo of Gualou-Xiebai.

关 键 词：引药 米酒 瓜蒌薤白 抗氧化活性 药效学 

分 类 号：R285.5[医药卫生—中药学]