一种含姜黄食品对去卵巢模型鼠骨组织的影响  

Effects of the food contained turmeric on bone tissue in ovariectomized rats

作　　者：陆宽[1,2] 张洁[3] 刘文炜[4] 霍昕[1] 刘建华 高玉琼[1] 冯发进 

机构地区：[1]贵州省生物技术研究开发基地,贵州贵阳550002 [2]贵州省科晖制药厂,贵州贵阳550011 [3]贵州医科大学,贵州贵阳550025 [4]贵阳中医学院第一附属医院,贵州贵阳550002 [5]贵州省中科院天然产物化学重点实验室,贵州贵阳550002

出　　处：《食品工业科技》2017年第11期334-339,共6页Science and Technology of Food Industry

基　　金：贵州省社会发展攻关计划项目[黔科合SY字(2014)3013];贵州省开发类科研院所技术开发研究专项资金项目[黔科合成字(2013)5028];贵州省科技人才建设项目[黔科合人才团队(2014)4011]

摘　　要：目的:探讨一种含姜黄食品对去卵巢模型鼠骨组织变化的影响。方法:采用双侧去卵巢法制备去卵巢诱发骨质疏松大鼠模型,将动物分为假手术组、模型对照组、含姜黄受试物组和不含姜黄受试物组,连续喂养90 d,测定各组大鼠体重变化、血清中骨保护素(Osteoprotegerin,OPG)及核因子Κ-β受体活化因子配体(Receptor Activator for Nuclear FactorΚ-βLigand,RANKL)水平、大鼠股骨长度以及骨组织形态学检测。结果:喂养90 d后,姜黄组大鼠体重增长趋势与假手术组较为接近(p>0.05)。姜黄组大鼠血清OPG水平、股骨长度、骨小梁厚度以及成骨细胞数显著高于模型对照组(p<0.01),RANKL水平、骨小梁间隙以及破骨细胞数显著低于模型对照组(p<0.01)。姜黄组与不含姜黄组比较,大鼠血清中OPG、骨小梁厚度高于不含姜黄组(p<0.05),RANKL、骨小梁间隙低于不含姜黄组(p<0.05);姜黄组大鼠股骨长度及成骨细胞数显著高于不含姜黄组(p<0.01),破骨细胞数显著少于不含姜黄组(p<0.01)。结论:实验结果表明,此含姜黄食品对去卵巢模型鼠骨组织退变有一定的抑制作用。Objective ：To explore the effects of the food contained turmeric on bone tissue in ovariectomized rats. Methods：The osteoporosis model was established by removing the ovaries of the rats.The animals were divided into sham group, model group, turmeric group ,and excluding turmeric group,continuous feeding 90 d.Then,the weight,femur length, and bone parameters of each group were measured.Blood was gained from the eyeballs of rats.Osteoprotegerin（OPG） and receptor activator for nuclear tactor K-β ligand （RANKL） level were measured by ELISA method. Results： The rats weight of turmeric group increased relatively close to the control group （p 〉 0.05 ）.The turmeric group had significant increase （p 〈 0.01 ）in the level of OPG, femur length,trabecular thickness,and the number of osteoblast, as well as significant decrease （p 〈 0.01 ） in the level of RANKL, trabecular space,and the number of osteoclast compared to the model group.The turmeric group had significant increase in the level of OPG and trabecular thickness（ both p 〈 0.05 ）, femur length and the number of osteoblast （both p 〈 0.01 ）, as well as significant decrease in the level of RANKL and trabecular space （both p 〈 0.05 ）, the number of osteoclast （p 〈 0.01 ）compared to the excluding turmeric group.Conclusion ：The results indicated that the food contained turmeric had inhibitory effect on bone tissue degeneration in ovariectomized rats.

关 键 词：姜黄 去卵巢模型鼠 骨质疏松 骨组织 

分 类 号：TS201.4[轻工技术与工程—食品科学]