生物分子相互作用技术检测黄曲霉毒素B_1  

Determination of Aflatoxin B_1 by Biomolecular Interaction Analysis

作　　者：许艳丽[1] 鲍蕾[1] 何晓霞 吴振兴[1] 静平[1] 曹文卿[1] 梁成珠[1] 

机构地区：[1]山东出入境检验检疫局,山东青岛266001 [2]青岛出入境检验检疫局

出　　处：《检验检疫学刊》2017年第2期6-8,共3页Journal of Inspection and Quarantine

基　　金：国家质检总局科技计划项目(2012IK174)

摘　　要：建立一种利用生物分子相互作用技术检测饲料中黄曲霉毒素B_1的方法。将黄曲霉毒素B_1抗体固化在芯片上,样品经甲醇-水提取、稀释后通过固化黄曲霉毒素B_1抗体的芯片,从而测定样品中的黄曲霉毒素B_1含量。方法检出限为1μg/kg,检测限为2μg/kg,回收率为67.0%-90.1%,相对标准偏差为6.57%-7.81%。该方法检测灵敏度高、检测时间短、操作简便易行。The concentration of aflatoxin B1 in feed was determined by the surtace plasmon resonance technique. The chip immobilized by anti-AFB1 body to determine the AFBL in the sample solution. The LOD of method is 1 μg/kg and LOQ is 2 μg/kg,the recoveries of AFB1 are 67.0%-90.1%,and the relative standard deviations are 6.57%-7.81%. This method is of high sensitivity,convenience operation.

关 键 词：生物分子相互作用 黄曲霉毒素 黄曲霉毒素B1抗体 

分 类 号：TS207.4[轻工技术与工程—食品科学]