基于纸质微流控芯片的农药检测系统  

Detection System for Pesticides with Paper-based Microfluidic Chip

作　　者：毛罕平[1] 左志强[1] 施杰[1] 杨宁[1,2] HUANG J S 严玉婷[1,4] 

机构地区：[1]江苏大学现代农业装备与技术教育部重点实验室,镇江212013 [2]江苏大学电气信息工程学院,镇江212013 [3]加州大学圣地亚哥分校信息理论与应用研究中心,圣地亚哥ca92126 [4]江苏大学化工化学学院,镇江212013

出　　处：《农业机械学报》2017年第5期94-100,共7页Transactions of the Chinese Society for Agricultural Machinery

基　　金："十二五"国家科技支撑计划项目(2014BAD08B03);国家自然科学基金重点项目(61233006);国家自然科学基金项目(31671584);江苏省农业科技自主创新资金项目(CXC1571033-02);江苏省高校优势学科建设工程项目(苏财教(2014)37号)

摘　　要：针对当前农药检测手段仪器复杂、成本昂贵等问题,提出了一种基于纸质微流控农药检测方法。设计了具有自动进样、混合反应、电化学检测等功能的纸质微流控芯片,采用石墨碳、Ag/AgCl材料以及结合化学交联法制备了环状结构的丝网印刷酶电极,并利用循环伏安法对制备的酶电极进行了电化学表征,构建了一套基于酶抑制法的集成酶电极纸质微流控农药检测系统。最后建立了酶抑制率与对硫磷浓度的数学模型,并测试了酶电极的性能。实验结果表明,酶电极具有良好的制备重复性、稳定性和线性度。抑制率与对硫磷浓度的负对数在1.0×10^(-7)~1.0×10^(-5)g/mL范围内呈良好的线性关系,线性回归方程为:I=158.82+21.11lg C,R^2为0.993,最低检出限为3.3×10^(-8)g/mL。所制备的酶电极微流控传感器抗干扰性较强,对对硫磷农药具有一定的选择性。加标回收率范围在95.8%~115.0%之间。In order to solve the problems relating to pesticides detection with the use of complex instruments in terms of high cost, tedious operation and low degree of automation, a new method for pesticides detection with paper-based microfluidic chip was provided. The paper-based microfluidic chip was designed and developed with automatic sample injection, hybrid reaction and electrochemical detection. Subsequently, the enzyme electrode of ring structure was prepared by chemical crosslinking method using graphite carbon and Ag/AgC1 materials and then characterized with electrochemistry cyclic voltammetry （CV）. The integrated enzyme electrode of the paper-based microfluidic detection system for pesticides was built based on enzyme inhibition mechanism. Finally, the linear model between inhibition rate and parathion pesticides concentration was set up and the performance of the enzyme electrode was tested. The results showed that the prepared enzyme electrode was of good repeatability, linearity and stability. Furthermore, a linear relationship was displayed between inhibition rate and parathion pesticides, which was represented by the equation I = 158.82 ＋ 21.11 lgC with good linear range of 1.0×10^-7～1.0×10^-5g/mL. The determination coefficient obtained was 0. 993 and the corresponding limit of detection was 3.3×10^-8g/mL. The enzyme electrode integrated on the paper-based microfluidic chip was robust with parathion pesticides which can not be interfered with other pesticides and substance with standard addition recovery rate of 95.8% -115.0%.

关 键 词：农药检测系统 酶抑制法 纸质微流控芯片 酶电极 电化学检测 

分 类 号：S220.1[农业科学—农业机械化工程] TH703[农业科学—农业工程]