一株高产辛酸乙酯地衣芽孢杆菌的诱变育种  

A Mutation Breeding of A Ethyl Caprylate High Producing Bacillus licheniformis Strain

作　　者：周金虎[1,2,3] 魏浩林 张明春 邓其锦 陈茂彬[1,2,3] 方尚玲[1,2,3] 

机构地区：[1]发酵工程教育部重点实验室(湖北工业大学),湖北武汉430068 [2]工业发酵湖北省协同创新中心,湖北武汉430068 [3]工业微生物湖北省重点实验室,湖北武汉430068 [4]湖北白云边酒业股份有限公司,湖北松滋434200

出　　处：《酿酒》2017年第3期20-24,共5页Liquor Making

基　　金："十三五"国家重点研发计划项目中的重点课题子课题(2016YFD0400500):酿造食品风味与品质调控及新型酿造技术创制

摘　　要：为了提高产辛酸乙酯地衣芽孢杆菌(Bacillus lincheni formis)的产酯能力,以前期分离得到的产辛酸乙酯地衣芽孢杆菌作为出发菌株,通过紫外线对该菌株进行诱变,筛选高产辛酸乙酯的突变株。结果显示,经诱变后筛选出一株发生正突变的菌株即DY-3,经发酵后通过气相色谱法测定其辛酸乙酯的产量较出发菌株提高了62.5%。In order to improve the production capacity of etheyl octanoate ester of bacillus licheniformis,In this paper we took the etheyl octanoat bacillus licheniformis which were produced from preliminary separation as a starting strain,through ultraviolet radiation on the strain mutagenesis, screening of high yield mutant strains of octanoic acid ethyl ester.The results indicated that after mutagenesis and screened strain positive mutated strain of DY-3.To determine its etheyl octanoate production after fermenting by gas chromatography.The production increased by 62.5% compare with the starting strain.

关 键 词：地衣芽孢杆菌 辛酸乙酯 诱变 气相色谱(GC) 

分 类 号：TS262.3[轻工技术与工程—发酵工程] TS261.15[轻工技术与工程—食品科学与工程]