Aurora-A高表达对食管癌细胞与细胞间黏附能力的影响  

Effect of Aurora-A overexpression on cell-cell adhesion of esophageal carcinoma cells

作　　者：贺春[1] 寇俊婷 孙雪娇[1] 任本洪 王晓霞[1] 

机构地区：[1]山西医科大学生物化学与分子生物学教研室,山西太原030001

出　　处：《中国生物制品学杂志》2017年第5期477-480,共4页Chinese Journal of Biologicals

基　　金：国家自然科学基金资助项目(30973401);中国博士后基金资助项目(2014M551058)

摘　　要：目的探讨Aurora-A高表达对食管癌KYSE150细胞与细胞间黏附能力的影响。方法体外培养人食管癌KYSE150细胞,在Lipofectamine 2000介导下,分别转染高表达质粒p EGFP-C1-Aurora-A及载体p EGFP-C1,同时设空白细胞对照组(未转染)。通过Western blot法检测人食管癌KYSE150细胞中Aurora-A的表达;采用细胞缓慢聚集及细胞分离试验观察Aurora-A高表达对细胞与细胞间黏附能力的影响。结果 Aurora-A高表达组可见相对分子质量约72 000的GFP-Aurora-A融合蛋白条带,而空载体对照组及空白细胞对照组未见该蛋白条带。Aurora-A高表达组、空白细胞对照组及空载体对照组细胞团数分别为(71.0±9.4)、(25.3±0.6)和(28.5±4.0)个,N_(TC)/N_(TE)值分别为0.162±0.026、0.087±0.016和0.080±0.008,Aurora-A高表达组细胞团数及NTC/NTE值明显高于空白细胞对照组及空载体对照组(P<0.05)。结论 Aurora-A高表达能显著降低食管癌细胞与细胞间的黏附能力,进而增加肿瘤细胞的恶性表型。Objective To investigate the effect of Aurora-A overexpression on cell-cell adhesion of esophageal carcinoma cells.Methods Esophageal carcinoma KYSE150 cells were cultured in vitro and transfected with plasmids pEGFP-C1-Aurora-A and pEGFP-C 1 in mediation of Lipofectamine 2000 respectively,using untransfected cells as blank control.The expression of Aurora-A was determined Western blot,while the effect of Aurora-A overexpression on cell-cell adhesion was observed by slow cell aggregation and cell dissociation assays.Results GFP-Aurora-A fusion protein with a relative molecular mass of about 72 000 was observed in KYSE150 cells transfected with plasmid pEGFP-C1-Aurora-A.However,no such a band was observed in the cells transfected with empty vector or those untransfected.The cell mass number in cells transfected with pEGFP-C1-Aurora-A and pEGFP-C1 and in the untransfected cells were （71.0 ＆#177; 9.4）,（25.3 ＆#177; 0.6） and （28.5 ＆#177; 4.0）,while the NTC/NTE values were （0.162 ＆#177; 0.026）,（0.087 ＆#177; 0.016） and （0.080 ＆#177; 0.008）,respectively,both of which were significantly higher in the cells transfected with pEGFP-Cl-Aurora-A than in those transfected with pEGFP-Cl and those untransfected （P 〈 0.05）.Conclusion Aurora-A overexpression inhibited the cellcell adhesion and increased the malignant phenotype of esophageal carcinoma cells.

关 键 词：AURORA-A 食管癌细胞 黏附能力 细胞缓慢聚集试验 细胞分离试验 

分 类 号：R735.1[医药卫生—肿瘤]