猴头菌丝固体培养物及胃乐宁片低聚糖部位的HPLC-ELSD指纹图谱  

HPLC-ELSD fingerprints of oligosaccharide sites from mycelia of Hericium erinaceum solid cultures and Weilening Tablets

作　　者：陈婷[1,2] 杨军辉[1,2] 李俊松[1,2] 陈坤 狄留庆[1,2] 郭青[4] 

机构地区：[1]南京中医药大学药学院,江苏南京210046 [2]江苏省中药高效给药系统工程技术研究中心,江苏南京210046 [3]南京老山药业有限公司,江苏南京210004 [4]江苏省食品药品检验所,江苏南京210008

出　　处：《中成药》2017年第6期1211-1216,共6页Chinese Traditional Patent Medicine

摘　　要：目的建立猴头菌Hericium erinaceus丝固体培养物及胃乐宁片低聚糖部位的HPLC-ELSD指纹图谱。方法样品水提液的分析采用Waters XBridgeTMAmide色谱柱(4.6 mm×150 mm,3.5μm);以乙腈-0.2%醋酸铵为流动相,梯度洗脱;体积流量1 mL/min;柱温40℃。结果 2种HPLC-ELSD指纹图谱分别有8个和9个共有峰,相似度分别为0.994~0.966和0.990~0.997,其中3个为甘露醇、乳糖和海藻糖,在各自范围内线性关系良好(r≥0.999 0),平均加样回收率95.08%~104.82%,RSD 1.12%~2.90%。结论该方法简便、快速、准确,可用于猴头菌丝固体培养物及胃乐宁片的质量控制。AIM To establish the HPLC-ELSD fingerprints of oligosaccharide sites from mycelia of Hericium erinaceum solid cultures and Weilening Tablets.METHODS The analysis of aqueous extract from samples was performed on a 80 ℃ thermostatic Waters XBridgeTM Amide column（4.6 mm×150 mm,3.5 μm）,with the mobile phase comprising of acetonitrile-0.2% ammonium acetate flowing at 1 mL/min in a gradient elution manner.RESULTS There were eight and nine common peaks in two HPLC-ELSD fingerprints with the similarties of0.994-0.966 and 0.990-0.997,respectively.Three of them were mannitol,lactose and trehalose,which showed good linear relationships within their own ranges（r≥0.999 0）,the average recoveries were 95.08%-104.82% with the RSDs of 1.12%-2.90%.CONCLUSION This simple and accurate method can be used for the rapid quality control of mycelia of Hericium erinaceum solid cultures and Weilening Tablets.

关 键 词：猴头菌丝 固体培养物 胃乐宁片 低聚糖 甘露醇 乳糖 海藻糖 HPLC-ELSD指纹图谱 

分 类 号：R284.1[医药卫生—中药学]