弱酸性电位水结合壳聚糖对银耳活性氧代谢的影响  

Influence of Slightly Acidic Electrolyzed Water and Chitosan Treatment on Activate Oxygen Metabolism of Tremellafuciformis

作　　者：陈岗[1] 詹永[1] 杨勇[1] 谢会川 石文娟 柴佳炎 罗杨[1] 

机构地区：[1]重庆市中药研究院,重庆400065 [2]重庆市银耳营养食品企业工程技术研究中心,重庆409003

出　　处：《天然产物研究与开发》2017年第5期747-755,共9页Natural Product Research and Development

基　　金：重庆市科技攻关项目(cstc2013yykfa80004);重庆市科技支撑示范工程项目(cstc2015jcsf-kjfp80014);重庆市社会事业与民生保障科技创新专项(cstc2016shmszx80077)

摘　　要：为解决新鲜银耳不耐贮藏的问题,对比研究了直接贮藏、壳聚糖涂膜、弱酸性电位水(slightly acidicelectrolyzed water,SAEW)浸泡清洗及SAEW清洗结合壳聚糖涂膜四种处理方法对新鲜银耳采后活性氧代谢相关生理特性变化的影响。研究结果表明:相对于直接贮藏,壳聚糖和SAEW处理对采后银耳具有一定的防腐保鲜作用,但在贮藏后期防腐能力均出现减弱。两种处理方式联合使用,提升了防腐保鲜效果,在10 d贮藏期内,微生物菌落总数、呼吸速率和细胞膜透性等指标维持在较低水平无较大波动,抗氧化酶SOD、POD和CAT酶活性始终处于较高水平,非酶抗氧化物As A和GSH含量降低速度显著低于其它实验组(P<0.05),表现出很好的贮藏效果。与单一使用壳聚糖或SAEW处理相比,二者结合使用能更有效地保持银耳的品质。本实验为新鲜银耳的防腐保鲜提供了一种新的技术途径。In order to solve the issues of intolerant preservation of Tremellafuciformis, the effects of different treatments on changes of physiological characteristics related to active oxygen metabolism of post-harvest T.fuciformiswere investigated by using four different processing methods on direct storage, chitosan coating, slightly acidicelectrolyzed water（SAEW） immersion cleaning and SAEW-assisted chitosan processing. The results showed that the processing of SAEW cleaning and chitosan coating can play a favorable preservation but the corrosion resistance appeared to weaken at later storage period. SAEW-assisted chitosan processing was superior to other treatments in some indexes. In the 10 d storage period, the total number of colonies, microbial respiration rate and biochemical index maintained at low levels but no greater volatility, the activity of antioxidant enzymes （SOD, POD and CAT） remained at a high level, the decreased rate of non-enzyme antioxidants（ AsA and GSH） was significantly lower than that of the other experimental group（P 〈 0.05 ）. The conclusion provided a new useful method for safer preservation of T. fuciformis.

关 键 词：弱酸性电位水 壳聚糖 银耳 活性氧代谢 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]